肝细胞生长因子和血管内皮生长因子在急性髓系白血病中的表达以及与血管新生的关系

Expression of hepatocyte growth factor and vascular endothelial growth factor in acute myeloid leukemia and their relationship with angiogenesis

目的探讨肝细胞生长因子（HGF）和血管内皮生长因子（VEGF）在急性髓系白血病（AML）中的表达及与AML血管新生的关系。方法应用酶联免疫吸附试验（ELISA）测定AML25例初发未治、17例完全缓解、16例未缓解、12例复发患者血清中HGF和VEGF的浓度，并与正常对照组比较。结果血清HGF的浓度：AML初发未治组[（1357．29±358．64）ng／L]、未缓解组[（1175．93±306．71）ng／L]、复发组[（1261．21±340．83）ng／L]患者均明显高于正常对照组[（232．62±99．13）ng／L]和缓解组[（256．65±94．32）ng／L]（F=78．35，P〈0．01）；血清VEGF的浓度：AML初发未治组[（253．84±49．14）ng／L]、未缓解组[（245．87±54．68）ng／L]、复发组[（264．75±62．52）ng／L]患者均明显高于正常对照组[（97．61±16．19）ng／L]和缓解组[（99．76±15．93）ng／L]（F=68．65，P〈0．01）；缓解组患者血清HGF和VEGF的浓度与正常对照组比较，差异均无统计学意义（P均〉0．05）。HGF和VEGF在AML中呈正相关（r=0．49，P〈0．05）。结论HGF和VEGF与AML的发生、发展密切相关，通过阻抗HGF治疗白血病有望成为新的治疗方法。

Objective To investigate the expression of hepatocyte growth factor （HGF）and vascular endothelial growth factor （ VEGF ） in acute myeloid leukemia （ AML ） and its significance in the progress of AML Methods Serum levels of HGF and VEGF in AML patients, including 25 de novo cases, 17 cases with complete remission（ CR）, 16 cases without remission and 12 cases with relapse, were measured by enzyme-linked immunosorbent assay （ ELISA ）. The data were compared with those of normal controls. Results The serum levels of HGF were（1357. 29±358. 64） ng/L in de novo patients, （1175.93 ±306. 71 ）ng/L in patients without remission and（ 1261.21± 340. 83 ） ng/L in patients with relapse, which were significantly higher than those in normal control（232. 62 ± 99. 13 ） ng/L and CR patients （ 256. 65± 94. 32 ） ng/L （ F = 78. 35, P 〈 0. 01 ）. The serum levels of VEGF were （ 253.84 ± 49.14 ） ng/L in de novo patients, （ 245.87 ± 54.68 ） ng/L in patients without remission and（ 264. 75 ±62. 52 ）ng/L in patients with relapse, which were significantly higher than those in normal control （ 97.61± 16. 19 ） ng/L and CR patients （ 99. 76± 15. 93 ） ng/L （ F = 68. 65, P 〈 0. 01 ）. Compared with de novo group, the serum levels of HGF and VEGF in CR patients decreased significantly but showed no significant difference from those in normal controls （P 〉 0. 05 ）. The expression of HGF and VEGF in acute myeloid leukemia was positively correlated with each other （ r = 0. 49, P 〈 0. 05 ）. Conclusion The expression of HGF and VEGF was closely related with the incidence and development of acute myeloid leukemia. Preventing HGF expression may be a possible strategy for leukemia therapy in the future.