叉尾斗鱼遗传多样性的RAPD分析

Analysis of genetic diversity of paradisefish Macropodus opercularis using RAPD markers

采用RAPD标记技术对叉尾斗鱼Macropodus opercularis 5个地理群体(南宁群体、桂林群体、龙岩群体、福州群体和广州群体)的遗传多样性进行了检测。结果表明:从40个随机引物中筛选出11个有效引物,对每条叉尾斗鱼的基因组DNA进行扩增,共扩增出139条片段;各群体的多态位点比例为26.44%～40.95%,Nei基因多样性指数为0.1042～0.1457,Shannon指数为0.1543～0.2176;而总体的多态位点比例、Nei基因多样性指数和Shannon指数分别高达84.89%、0.3110和0.4606。研究表明,叉尾斗鱼的遗传变异主要来自群体间,而群体内部的遗传多样性水平较低。

Random amplified polymorphic DNA（RAPD） technique was employed to detect the genetic variations in paradisefish Macropodus opercularis populations distributed in five geographical colonies.In this study,11 effective primers out of 40 random primers were selected to amplify genomic DNA,resulting in 139 repeatable RAPD bands.The 5 populations were shown to have polymorphism frequency of 26.44%-40.95%,Shannon genetic diversity index of 0.1042-0.1457 and Nei genetic diversity of 0.1543-0.2176.On the average,all the population showed the frequency of polymorphism of 84.89%,Nei genetic diversity of 0.3110 and Shannon genetic diversity index of 0.4606,indicating that the genetic variation of the paradiselfish was not from the intra-populations but from inter-populations.