黄腊果果皮化学成分的分离与鉴定

Isolation and identification of chemical constituents from peels of Stauntonia brachyanthera Hand. Mazz

目的研究黄腊果果皮的化学成分。方法采用反复硅胶柱色谱法、Sephadex LH-20柱色谱法、重结晶和HPLC等方法进行分离纯化,并通过NMR技术鉴定其化学结构。结果从黄腊果中分离得到8个化合物,分别鉴定为:3-羟基-2-甲基-4-吡喃酮(3-hydroxy-2-methyl-4-pyrone,1)、芥子醛(sinapaldehyde,2)、2-甲基-4-吡喃酮-3-O-β-D-吡喃葡萄糖苷(2-methyl-4-pyrone-3-O-β-D-glucopyr-anoside,3)、3,5-二甲氧基-4-羟基苯甲酸-7-O-β-D-葡萄糖苷(3,5-dimethoxyl-4-hydroxybenzoic acid-7-O-β-D-glucopyranoside,4)、胡萝卜苷(daucosterol,5)、β-谷甾醇(sitosterol,6)、3-O-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖-30-降甲基齐墩果-12,20(29)-二烯-28-羧酸(3-O-α-Lrhamnopyrano-syl-(1→2)-α-L-arabinopyranosyl-30-norolean-12,20(29)-dien-28-oic acid,7)、3-O-β-D-吡喃葡萄糖-(1→3)-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖-30-降甲基齐墩果-12,20(29)-二烯-28-羧酸(3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-D-glucopyranosyl-(1→3)-α-L-arabi-nopyranosyl-30-norolean-12,20(29)-dien-28-oic acid,8)。结论化合物1-4为首次从木通科植物中分离得到,化合物6-8为首次从黄腊果中分离得到。

Objective To study the chemical constituents from peels of Staunton iabrachyanthera Hand.Mazz..Methods The compounds were isolated by silica gel column,SephadexLH-20 column,recrystallization and HPLC method,their structures were confirmed on the basis of NMR analysis.Results Eight compounds were isolated and identified as 3-hydroxy-2-methyl-4-pyrone（1）,sinapaldehyde（2）,2-methyl-4-pyrone-3-O-β-D-glucopyranoside（3）,3,5-dimethoxyl-4-hydroxybenzoic acid-7-O-β-D-glucopyranoside（4）,daucosterol（5）,sitosterol（6）,3-O-α-L-rhamnopyranosyl-（1→2）-α-L-rhamnopyranosyl-30-norolean-12,20（29）-dien-28-oic acid（7）,3-O-β-D-glucopyranosyl-（1→3）-α-L-rhamnopyranosyl-（1→2）-α-L-arabinopyranosyl-30-norolean-12,20（29）-dien-28-oic acid（8）.Conclusions compounds 1-3 and 4 are firstly isolated from the Laidizabalaceae,while compounds 6-8 are isolated from this herb for the first time.