探讨γ-谷氨酰基转移酶冰冻混合人血清候选标准物质的制备和稳定性

Preparation and stability of the mixed human candidate reference standard serum for gamma-glutamyltransferase

目的探讨γ-谷氨酰基转移酶(gamma-glutamyltransferase,GGT)冰冻混合人血清候选标准物质制备方法的可行性,同时观察并分析其在-80℃保存的稳定性。方法对收集的GGT血清按5个浓度水平进行混合过滤分装,同时分别在0、7、30、60、120、180、330、360、390、750 d测定GGT冰冻混合人血清候选标准物质,采用线性回归方差分析对测定结果进行稳定性检验,评价其稳定性。结果在750 d以内,C1～C5线性回归方差分析的P值均>0.05(C1～C5测定结果 P值分别为0.886,0.753,0.585,0.571,0.945)。结论本实验室采用的GGT冰冻混合人血清候选标准物质制备方法简便易行,且候选人血清标准物质在750 d内稳定性良好。

Objective To investigate the feasibility of freezing admixing human serum which was the selected standard materials of gamma-glutamyltransferase（GGT）,and to observe and analyze their stability at-80 ℃.Methods The GGT serum which had been collected was mixed,filtered and subpackaged according to five levels.Then the selected standard materials were measured at 0,7,30,60,120,180,330,360,390 and 750 days.In order to evaluate the stability,the results were statistic analyzed by linear regression.Results In the 750d,the P of the linear regression about C1~C5 was more than 0.05（the P of C1~C5 was 0.886,0.753,0.585,0.571 and 0.945）.Conclusion The preparation method of the freezing admixing human serum which was the selected standard materials of GGT was simple and convenient,otherwise the stability of the selected standard materials was well in 750 days.