核黄素光化学作用灭活血液中淋巴细胞效果研究

Study on the Inactivation of Lymphocytes in Blood Using Riboflavin Photochemical Treatment with Visible Light

目的探讨核黄素联合可见光照射对诱导血液中淋巴细胞凋亡的效果。方法利用流式细胞仪检测经核黄素光化学法处理的淋巴细胞的凋亡。结果淋巴细胞凋亡数量明显增加。结论核黄素联合可见光照射可有效诱导淋巴细胞凋亡,凋亡是淋巴细胞减少的主要原因。

Objective To explore the effect of a photochemical method on inducing the apoptosis of lymphocytes using riboflavin plus visible light.Methods The apoptosis of lymphocytes was detected by flow cytometer after the processing of riboflavin photochemical treatment.Results The number of lymphocyte apoptosis increased obviously.Conclusion Riboflavin photochemical treatment with visible light can induce the apoptosis of lymphocytes effectively,apoptosis is the main reason for the reduced lymphocytes.