摘要
目的研究125I标记抗人ADAM15多克隆抗体(125I-anti-ADAM15抗体)在荷人胃癌裸鼠体内的生物分布。方法以氯胺T法制备125I-anti-ADAM15抗体并测定其在生理盐水和人血清中的稳定性。经荷胃癌裸鼠模型尾静脉注入125I-anti-ADAM15抗体后1、4、8、24和48 h对裸鼠进行SPECT平面显像,采用感兴趣区(ROI)技术进行半定量分析,计算肿瘤与非瘤组织的放射性计数比值(T/NT)和肿瘤与裸鼠全身的放射性计数比值。于48 h显像后处死荷瘤裸鼠,取心、肺、甲状腺和肿瘤等多个脏器组织称重并测量其放射性计数,计算各组织的每克组织百分注射剂量率(%ID/g)。结果125I-anti-ADAM15抗体标记率为(75.16±9.43)%,纯化后放射化学纯度可达(99.44±0.21)%。经尾静脉注入125I-anti-ADAM15抗体后,随时间的延长,T/NT逐渐增高。在48 h时肿瘤清晰显影,T/NT可达3.84±0.43。结论125I-anti-ADAM15抗体在荷胃癌裸鼠体内具有肿瘤靶向定位能力,能获得良好的放射免疫显像图像。
Objective To investigate the biodistribution of Iodine-125 labeled anti-human ADAM15 polyclonal antibody in nude mice bearing human gastric carcinoma xenografts. Methods The anti-human ADAM15 polyclonal antibody was labeled with 1-125 using Chloramine-T method. The labeling efficiency and radiochemical purity of 125I-anti-ADAM15 antibody were measured. The SPECT plannar imaging of nude mice bearing gastric carcinoma xenografts were performed at 1, 4, 8, 24, and 48h post-injection and the biodistribution of 125I-anti-ADAM15 antibody was measured at 48 h after injection. Results The labeling efficiency of 125I-anti-ADAM15 antibody was （75.16±9.43 ） % and its radiochemical purity was （99.44±0.21 ） %. Tumors could be cleanly visualized in SPECT planar images, and the radioactivity ratio of tumor to non-tumor tissue was 3.84±0.43 at 48 h post-injection. Conclusion 125I-anti-AD- AMI5 antibody can target the gastric carcinoma in vivo, and provide good radioimmunoimages.
出处
《苏州大学学报（医学版）》
CAS
2012年第2期166-169,共4页
Suzhou University Journal of Medical Science
基金
苏州市国际合作基金资助项目(SWH0926)
