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MicroRNA155通过下调清道夫受体表达抑制巨噬细胞泡沫化形成 被引量：11

MicroRNA-155 Inhibits Macrophagic Foam Cell Formation through Downregulating Expression of Scavenger Receptors
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摘要 【目的】探讨microRNA-155(miR-155)对巨噬细胞泡沫化过程的影响及机制。【方法】实时定量PCR检测miR-155的表达,Western Blot方法检测巨噬细胞A类清道夫受体SR-A和B型清道夫受体CD36的表达,激光共聚焦显微镜观察miR-155对THP-1结合、摄取DiI标记氧化型低密度脂蛋白(DiI-oxLDL)能力的影响。【结果】80μg/mL的氧化型低密度脂蛋白(ox-LDL)时间依赖性地诱导巨噬细胞miR-155表达上调。过表达miR-155抑制SR-A和CD36的表达,同时巨噬细胞结合、摄取DiI-oxLDL的能力明显降低。而反义-miR-155则明显上调SR-A和CD36的表达,同时巨噬细胞结合、摄取DiI-oxLDL的能力明显增强。【结论】MiR-155通过降低巨噬细胞SR-A和CD36的表达抑制巨噬泡沫细胞的形成。 【Objective】 To study the effect of microRNA-155（miR-155） on macrophagic foam cell formation.【Methods】 Real-time RT-PCR was used to test the expression of microRNA-155.Western blot was used to determine the expression of scavenger receptors SR-A and CD36 in THP-1 macrophages.Receptor-specific binding and uptake of DiI-labeled ox-LDL（DiI-oxLDL） were examined by laser scanning confocal microscope.【Results】 In THP-1 cells,80 ug/mL oxidized low density lipoprotein（ox-LDL） induced up-regulation of miR-155 in a time-dependent manner.Ad-miR-155 transfection decreased the expression of SR-A and D36,and the uptake and binding of THP-1 cell with DiI-oxLDL.In contrast,miR-155 inhibitor increased the expression of SR-A and CD36,and the uptake and binding of THP-1 cell with DiI-oxLDL..【Conclusion】 MicroRNA-155 inhibits macrophagic foam cell formation through decreasing the expression of SR-A and CD36.
作者 尚菲 曾德意 杨慧 黄琳燕 刘捷 吕晓飞 关永源 周家国
机构地区 中山大学中山医学院 广东省医学科学院//广东省人民医院医学研究中心
出处 《中山大学学报（医学科学版）》 CAS CSCD 北大核心 2012年第2期156-162,共7页 Journal of Sun Yat-Sen University:Medical Sciences
基金 国家自然科学基金(30873060,30973536) 全国百篇优秀博士论文专项基金(200773) 中央高校基本科研业务费专项资金(09ykpy81)
关键词 MIR-155 动脉粥样硬化 THP-1巨噬细胞 清道夫受体 泡沫化 microRNA-155 atherosclerosis THP-1 cell foam cell Scavenger receptor
分类号 R966 [医药卫生—药理学]
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参考文献14

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共引文献17

同被引文献83

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引证文献11

二级引证文献58

中山大学学报（医学科学版）
2012年 第2期

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