手足口病患儿血清IL-6、IL-10、IL-17水平的变化及其临床意义

Change and Clinical Significance of Serum IL-6,IL-10,and IL-17 level in Children with Hand,Foot,and Mouth Disease

【目的】探讨手足口病(HFMD)患儿外周血白介素-6(IL-6)、白介素-10(IL-10)、白介素-17(IL-17)水平及其在HFMD发展过程中的变化。【方法】本实验采用酶联免疫吸附实验(ELISA)双抗体夹心法测定19例HFMD患儿(急性期、恢复期)及同期20例健康体检儿童血清IL-6、IL-10、IL-17的含量;并对HFMD患儿急性期、恢复期及健康儿童进行两两比较,了解IL-6、IL-10、IL-17在HFMD发展过程中的变化。【结果】1、手足口病患儿急性期IL-6、IL-10及IL-17水平显著高于健康对照组(P<0.01);恢复期IL-6及IL-17水平与对照组比较无显著差异(P>0.05)。而IL-10水平仍高于健康对照组(P<0.01)。2、急性期IL-6与IL-10呈负相关关系(P<0.05);IL-6与IL-17呈正相关关系(P<0.01);IL-10与IL-17呈负相关关系(P<0.05)。恢复期:IL-6与IL-10呈负相关关系(P<0.05);IL-6与IL-17呈正相关关系(P<0.01);IL-10与IL-17无相关关系(P>0.05)。【结论】手足口病患儿急性期炎症细胞因子(IL-6、IL-17)水平显著升高,而抑制性细胞因子IL-10水平升高持续到恢复期。

【Objective】 To study the changes and clinical significance of serum interleukin（IL）-6,IL-10,and IL-17 levels in the children with hand foot and mouth disease（HFMD）.【Method】 The serum concentration of IL-6,IL-10,and IL-17 in 19 cases of HFMD children（acute stage and convalescence） and 20 cases of healthy controls were measured by ELISA.The cytokines levels were compared.【Results】 1.Compared with healthy controls,the levels of IL-6,IL-10,and IL-17 in acute stage of HFMD were significantly increased in HFMD children（P 0.01）.There was no significant difference of the levels of IL-6 and IL-17 between the convalescence of HFMD and healthy children（P 0.05）,However the levels of IL-10 in convalescence of HFMD were higher than healthy children（P 0.01）.2.Spearman correlation analysis revealed that IL-6 and IL-17 were negatively correlated with IL-10（P 0.05）;IL-6 positively correlated with and IL-17 in acute stage（P 0.01）.In convalescence IL-6 was negatively correlated with IL-10（P 0.05）,positively correlated with IL-17（P 0.01）;IL-10 and IL-17 have no correlative relation（P 0.05）.【Conclusions】 1.The levels of IL-6 and IL-17 were significantly increased in acute stage of HFMD children.2.The persistent elevation of serum IL-10 level was found in HFMD patients after convalescence.