4-氯酚对厌氧颗粒污泥产甲烷活性的影响

Influence of 4-chlorophenol on activity of methane-producing microorganisms in anaerobic granular sludge

利用取自ABR反应器的厌氧颗粒污泥,以葡萄糖为共基质,测定了不同浓度4-氯酚对厌氧污泥产甲烷微生物的影响以及活性恢复情况。试验结果表明:4-氯酚对厌氧颗粒污泥产甲烷活性具有较强的抑制作用,当4-氯酚的质量浓度为300 mg/L时,抑制作用最小;并且随着4-氯酚浓度的继续提高,厌氧颗粒污泥的产甲烷活性显著下降。当4-氯酚的质量浓度为400、500、600 mg/L时,相应的抑制程度为31%、68%、54%。4-氯酚对厌氧生物降解葡萄糖反应的抑制作用,在低浓度时发生在反应后期,高浓度时发生在反应初期。同时在恢复试验中不同浓度的4-氯酚对产甲烷的抑制作用仍存在,但有所降低。

With anaerobic granular sludge from an anaerobic baffled reactor（ABR） as the research object and glucose as the co-substrate,the influence of 4-chlorophenol concentration on the methane-producing micro-organisms in anaerobic granular sludge was investigated.The test results showed that： 4-chlorophenol had a strong inhibition effect on the activity of methane-producing microorganisms in anaerobic granular sludge,the slightest degree of inhibition appeared when the mass concentration of 4-chlorophenol was 300 mg/L;and then,with the continuous increase of the 4-chlorophenol concentration,the methane-producing activity of anaerobic granular sludge decreased obviously;when it increased to 400,500 and 600 mg/L,the inhibition rate were 31%,68% and 54% respectively.The inhibition effect of 4-chlorophenol on the anaerobic biological degradation of glucose happened in the later stage of the reaction when the concentration was low,and in the early stage of the reaction when the concentration was high.In the recovery test carried out simultaneously,the inhibition effect of 4-chlorophenol with different concentrations on the production of methane was still exist;however,the inhibition degree was decreased.