摘要
将拟南芥基因AtGLR1.4启动子驱动的AtGLR1.4基因与绿色荧光蛋白(GFP)基因融合后,利用根瘤农杆菌介导瞬时转化法(Fast Agro-mediated Seedling Transfomation,FAST)浸染拟南芥幼苗,对其进行亚细胞定位的研究。转基因植株通过激光共聚焦扫描显微镜的观察,发现GFP绿色荧光在叶片表皮细胞的细胞膜上特异表达,表明At-GLR 1.4蛋白定位于细胞质膜上,为其后续的功能研究提供了线索。
In this study, the native promoter of AtGLRI. 4 was utilized to drive the expression of AtGLR1.4 fused with a report gene Green Fluorescent Protein（GFP） and then used to transiently transform Arabidopsis young seedlings by the fast agro-mediated seedling transformation（FAST） method. The expression of GFP would indicate the subcellular＇localization of AtGLR1.4. Confocal microscopy analysis of the transformed plants showed that the GFP signal was specifically detected on the plasma membrane of leaf epidermal plasma membrane of leaf epidermal cells. It suggested that AtGLRI. 4 was located on plasma membrane.
出处
《生物学杂志》
CAS
CSCD
2012年第2期51-54,共4页
Journal of Biology
基金
浙江省自然科学基金(编号:Z306401)
宁波市自然科学基金(编号:2006A610074)资助
