正常顺产儿与早产儿人巨细胞病毒和风疹病毒脐带血清抗体检测分析

The analysis of human cytomegalovirus and rubella virus antibody with healthy birth neonates and premature neonates

通过间接酶联免疫法检测178份新生儿(正常顺产儿为114例,早产儿64例)脐带血血清中人巨细胞病毒(human cytomegalovirus,HCMV)和风疹病毒(rubella virus,RV)IgG和IgM抗体,并分析所测结果与临床表现的相关性。结果表明,178例新生儿脐带血血清中HCMV-IgG阳性标本为168例(94.38%),HCMV-IgM阳性标本为1例(0.56%);RV-IgG阳性标本为119例(66.85%);RV-IgM阳性标本为1例(0.56%)。其中,正常顺产儿脐带血中HCMV-IgM和RV-IgM阳性率均为0.87%(1/114),HCMV-IgG阳性率为94.73%(108/114),RV-IgG阳性率为61.40%(70/114),HCMV和RV IgG两者均阳性者为55.26%(63/114);早产儿HCMV-IgM和RV-IgM均为阴性(0/64),HCMV-IgG阳性率为93.75%(60/64),RV-IgG阳性率为76.56%(49/64),HCMV和RV IgG两者均阳性者为70.31%(45/64)。早产儿与正常顺产儿比较,早产儿的RV-IgG阳性率和HCMV和RV-IgG两者均阳性者均高于正常顺产儿,且差异有统计学意义(P<0.05)。可见,HCMV感染率较高,至今仍无有效的HCMV疫苗,应加大疫苗研发力度。所查新生儿RV-IgG阳性率为66.48%,提示中国33%以上的育龄期妇女有在孕早期暴露感染的机率,国家有必要加大该种疫苗的接种力度。

To analyze the serum-positive rate of rubella virus and human cytomegalovirus in neonatal babies born district and its rela- tionship with clinical manifestation of premature children, a total of 178 neonatal serum including 114 umbilical cord blood sera and 64 premature children sera between Nov 2010 to Apr 2011, the Italian DIESSE HCMV IgG, IgM and RV IgG, IgM ELISA test kit were applied for detection of the sera. The neonatal seropositivity rates were calculated by statistical analysis. In 178 neonatal serum, 168 cases（94. 38% ） were HCMV-lgG positive, 1 case（0. 56% ） was HCMV-IgM positive; 119 cases（66. 85% ） were RV-IgG positive; all cases were RV-IgM negative. In 114 umbilical cord blood sera, HCMV-IgM positive rate was 0. 87% （1/1 I4） ; RV-IgM positive rate was 0. 87 % （1/114 ）, HCMV-lgG positive rate was 94. 73 % （108/114 ）, RV-IgG-positive rate of 61.40% （70/114）, both HCMV and RV-IgG positive rate were 55.26% （63/114）; preterm neonatal HCMV-IgM positive rate was 0. 00% （0/64）; RV-IgM positive rate was 0. 00% , HCMV-IgG positive rate was 93.75% （60/64） , RV-IgG positive rate was 76. 56% （49/64）. At the same time both HC-MV and RV IgG positive rate were70. 31% （45/64）. Compared the seropositive rates of premalure neonates with those of natural healthy birth neonates, RV-IgG-positive rate and both HCMV and RV IgG positive rate, preterm neonates was higher than natural healthy birth neonates, and the difference was statistical significant（ P 〈0. 05 ）. There is still no effective vaccine of HCMV.