新生儿败血症与白介素-8水平关系的研究

Study on the relationship between interleukin - 8 and neonatal sepsis

采用双抗体夹心ELISA法检测65例新生儿败血症血清白介素-8(IL-8)水平,并测定了白介素-6(IL-6)、一氧化氮(NO)、白细胞(WBC)及嗜中性多形核白细胞(PMN)计数、血小板计数(PLT)和C-反应蛋白(CRP)。结果血清IL-8水平在败血症组(中位数504ng/L)显著高于非败血症组(中位数<156ng/L)及正常对照组(中位数<156ng/L),急性期高于恢复期;它与IL-6、WBC、PMN、及NO正相关,与PLT及CRP无相关;与败血症的病情、并发休克、多器官功能衰竭等也密切相关。提示IL-8是新生儿败血症的重要炎症介质,对观察疗效及判断预后有一定意义。

To explore the relationship between neonatal sepsis and interleuking - 8 (IL - 8) which is an activator, chemotaxis and regulator, serum interleukin - 8 (IL - 8), Interleukin - 6 (IL - 6), nitric oxide (NO), white blood cell (WBC), polymorphonuclear neutrophil leukocyte (PMN), platelet (PLT) and C-reaction protein (CRP) were measured in 65 sepsis neonates and 41 non -sepsis neonates. The results showed that the level of IL - 8 (median 504ng/L) in the sepsis group was significant higher than that in the nonsepsis group (median <156ng/L) and the normal control group (median < 156ng/L), respectively. The level of IL - 8 in acute phase was higher than that in convalescent phase in the sepsis group. It was noticed that the level of IL - 8 was positively correlated with some laboratory findings such as IL - 6, WBC, PMN and NO, the clinical sitation, shock was complicated by sepsis, and multi -organic failure (MOF). It is suggested that IL - 8 as an inflammatory mediator play an important role for judging therapeutic effect and prognosis of neonatal sepsis.