黄芩茎叶总黄酮预处理对大鼠缺血再灌注脑皮质微血管构筑和脂质过氧化损伤的保护作用

Protective effect of scutellaria baicalensis stem-leaf total flavonoid on the injury of microvascular architecture and lipid peroxidation induced by cerebral ischemia reperfusion in rats

目的：探讨黄芩茎叶总黄酮（SSTF）预处理对脑局灶性缺血再灌注大脑皮质的保护作用及其机制。方法：线栓法制备局灶性脑缺血再灌注模型，造模成功后24h测脑梗死体积，单宁酸一氯化铁媒染法显示大脑皮质微血管，并用Mivnt图像分析系统对皮质微血管密度（MVD）及微血管面积密度（MVA）进行定量分析；检测脑皮质组织中超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量。结果：SSTF预处理可改善术后大鼠的MVD和MVA降低因缺血再灌注引起的脑皮质组织MDA含量增加，提高SOD活力。结论：SSTF预处理可改善脑皮质微血管形态结构，抵制脂质化过氧化反应，减轻自由基损害，对缺血再灌注损伤脑组织具有保护作用。

Objective： To study the protective effects and mechanisms of scutellaria baicalensis stem-leaf total flavonoid （SSTF） on focal cerebral ischemia reperfusion injury in rats. Methods： The model of focal cerebral ischemia reperfusion was duplicated in rats using the suture embolic method. Brain infarcted volume was observed. TA-FE method was applied to observe the microvascular architecture of the cerebral cortex, and Mivnt image analysis system was used to analyze the mi- crovessel density （MVD） and the microvessel area density （MVA） of the cerebral cortex quantitatively~ The contents of malondialdehyde （MDA） and activity of superoxide dismutase （SOD） in the cerebral cortex were measured 24 hours after cere- bral isehemia reperfusion. Results： Compared with the ischemia reperfusion group, SSTF pretreatment reduced the brain infarcted volume, improved MVD and MVA, decreased the content of MDA and increased the activity of SOD in the cerebral cortex induced by ischemia reperfusion. Conclusion： SSTF pretreatment can improve microvascular architecture, inhibit lipid peroxidation and attenuate the oxygen free radicals mediated damage to the brain. There is a protective effect on the cerebral cortex with focal cerebral ischemia reperfusion in rats.