尖吻蝮蛇毒抑瘤组分Ⅰ对小鼠肺癌LLC细胞增殖的抑制作用

Effects of anti-tumor component Ⅰ from Agkistrodon acutus venom on cell proliferation of mouse Lewis lung cancer

目的:研究尖吻蝮蛇毒抑瘤组分Ⅰ(AAVC-Ⅰ)对小鼠肺癌LLC细胞增殖抑制的影响。方法:以小鼠肺癌LLC细胞为研究对象,采用显微镜观察细胞形态学的变化、MTT法检测药物敏感性、DNA ladder法检测细胞凋亡。结果:AAVC-Ⅰ对LLC细胞有较强的增殖抑制作用,抑制程度随药物浓度的增加而增强,其IC50为43.823μg/ml(P<0.05)。琼脂糖电泳观察到细胞DNA的片段化。结论:AAVC-Ⅰ可抑制LLC细胞增殖,诱导其凋亡。

Objective：To investigate the effects of anti-tumor component Ⅰ from Agkistrodon acutus venom（AAVC-Ⅰ） on the cell proliferation and apoptosis of mouse Lewis lung cancer（LLC）.Methods：Cell line of LLC was obtained and observed for the morphological change with invert microscope and detected by thiazolyl blue tetrazolium bromide（MTT） for the cell sensitivity to AAVC-Ⅰ.DNA ladder method was used to examine the cell apoptosis.Results：AAVC-Ⅰ produced strong inhibition on LLC cells in vitro,and the cyto-inhibition was enhanced with drug concentration,with a IC50（50% concentration of inhibition） of 43.823 μg/ml（P0.05）.In addition,DNA fragments were seen using agarose gel electrophoresis.Conclusion：AAVC-Ⅰ can inhibit LLC value and facilitate apoptosis of the cell line.