原发性肝癌中血管生成拟态的表达和预后相关性分析

The significance of vasculogenic mimicry expression and relevance with pathological grade,clinical stage and cirrhosis in primary liver cancer

目的:探讨原发性肝癌中是否存在血管生成拟态(vasculogenic mimicry,VM),进一步探究VM在原发性肝癌中与肝硬化、临床分期、病理分级、组织分型的关系及预后相关性分析。方法:收集142例经手术治疗、临床及随访资料完整的原发性肝癌患者的病理蜡块,重新切片,采用CD31、PAS双重染色观察VM的表达情况,分析VM表达的临床意义。结果:在原发性肝癌和正常肝组织中VM阳性率分别为38.7%(55/142)和0%(0/15)(P<0.01);VM的表达与原发性肝癌的临床分期、肿瘤大小、肝细胞癌Edmondson分级呈正相关(P<0.05);VM的表达与是否伴有肝硬化相关(P<0.05);VM阳性组与阴性组12个月、24个月、36个月的生存率分别为46%/72%,35%/52%,19%/44%,差异有统计学意义。结论:原发性肝癌中存在VM;缺血、缺氧可诱导VM的表达;表达VM者临床分期更晚;肝硬化可增加VM的表达;表达VM者生存率更低。

Objective：To explore whether vasculogenic mimicry（VM） exists in primary liver cancer or not and to study the formational features and clinical significance of VM.Methods：The complete clinical and pathological data of 142 cases of PLC were collected for the study.The expression of VM were observed by immunohistochemical and histochemical double staining of CD34 and PAS to analyze the expression of VM according to the clinical significance message of patients.Results：The positive rates of VM in PLC and normal liver tissue were 38.7 and 0.0.The difference had a higher statistical significance（P0.01）;There was statistical significance in the expression of VM among clinical stage,tumor size,Edmondson stage of HCC（P0.05）;The VM expression was significantly up-regulated in HCC with cirrhosis（P0.05）.Conclusion：The vasculogenic mimicry exists in Primary liver cancer,which is an unfavorable factor influencing prognosis of the patients with primary liver cancer..