烧伤创面成纤维细胞周期、羟脯氨酸和胶原比例动态变化

Dynamic changes in dermal cell cycles, contents of hydroproline and ratios of collagen type Ⅰ/Ⅲ in the burned wound

目的 探讨深Ⅱ度烧伤创面愈合规律。 方法 通过 4 8只大鼠深Ⅱ度烧伤模型 ,动态观察烧伤后不同时间点创面成纤维细胞增殖周期、羟脯氨酸 (OHP)含量和Ⅰ /Ⅲ型胶原比值变化及病理组织学改变。 结果 伤后 10d真皮细胞s期细胞百分比达高峰 ,伤后 2 1d仍显著高于正常对照组。OHP含量伤后逐渐增加 ,伤后 14d达高峰 ,伤后 2 1d仍显著高于正常对照组。伤后Ⅰ /Ⅲ型胶原比值逐渐降低 ,并显著低于正常对照组 ,病理切片观察伤后 3～ 10d明显可见白细胞浸润带 ,伤后 14d可见上皮细胞自创缘及坏死组织界面爬行生长。 结论 深Ⅱ度烧伤创面伤后 3～ 10d以炎症反应为主。伤后 10～ 14d主要表现为成纤维细胞增殖和新生胶原合成 ,伤后 2 1d真皮的修复尚未结束。伤后 14～ 2 1d主要为表皮细胞增殖和迁移。

Objective To study the dynamic changes in the dermal cell cycles contents of hydroproline and ratios of collagen type Ⅰ/Ⅲ in the burned wound. Methods The dermal cell cycles, contents of hydroproline, ratios of collagen typeⅠ/Ⅲ and pathologic changes were dynamically observed in 48 deep partial burn rats(TBSA10%), attempting to investigate the process of deep partial burn wound healing. Results The percentage of S phase reached the highest point on PBD 10. On PBD21,it was still significantly higher than that of normal group. OHP increased gradually after burn, and peaked on PBD14. On PBD21, the level of OHP was still significantly higher than that of normal group. The ratios of collagen type Ⅰ/Ⅲ decreased gradually and significantly lower than those of the normal group after burn. The strips of WBC infiltrating into wound were observed on PBD3, 7 and 10. On PBD 14, a large number of regenerative epidermal cells crept over wound surface. Conclusions The inflammation is obvious from PBD3 to PBD10. The proliferation of dermal cell and the synthesis, deposition of new collagen are predominant from PBD10 to PBD14, and the repair of dermis is not complete on PBD21. The proliferation and migration of epidermal cells are major activities from PBD14 to PBD21.