IL-2和γ-IFN对晚期肝癌患者淋巴细胞粘附肿瘤细胞能力的影响

Effects of recombinant IL- 2 and γ- IFN on the ability of lymphocyte imuneadhering tumor cells in terminal hepatocellular carcinoma

目的 研究重组 ＩＬ－ ２（ｒＩＬ－２）和 γ－ＩＦＮ对晚期肝癌患者淋巴细胞粘附肿瘤细胞能力的影响。方法 晚期肝癌患者２４例，用 ｒＩＬ－２和 γ－ＩＦＮ预培养患者外周血淋巴细胞 ４ｈ，采用肿瘤淋巴细胞花环试验测定淋巴细胞粘附肿瘤细胞直接接触效应与活性．结果 ｒＩＬ－２对正常人和肝癌患者的淋巴细胞免疫粘附肿瘤能力都有明显的的提升效应，但单独使用 ｒＩＬ－２对肝癌患者淋巴细胞免疫粘附肿瘤细胞的提升效应较差，ｒＩＬ－２与γ－ＩＦＮ联合使用可显著增强淋巴细胞对肿瘤细胞的免疫粘附功能．结论 肝癌患者淋巴细胞免疫粘附肿瘤细胞能力明显低下，ｒＩＬ－２与γ－ＩＦＮ联合使用具有协同刺激肝癌患者淋巴细胞活性之作用．

Objective To study the effects of rIL -2 and γ-IFN on the ability of lymphocytes immune -adhering tumor cells in terminal hepatocellular carcinoma. Methods Peripheral blood lymphocye of 21 cases with terminal hepatocellular carcinoma was imcubated for 4 hours with rIL-2 or rIL-2 and γ- IFN before expriment and the ability of lymphocytes immune-adhering tumor cells was examined by the rate of tumor lymphocyte rosette. Results The ability of lymphocytes immune-adhering tumor cells was significantly enhanced by incubating with rIL-2 and γ-IFN in both of healthy individuals and the diseases of hepatocellular carcinoma. But the rosette rate of the diseases of hepatocellular carcinoma was obviously lower than that of healthy imdividuals. Conclusions The ability of lymphocytes immune-adhering in hepatocellular carcinoma can be obviously enhanced by using rIL-2 to gether with γ-IFN.