绿僵菌发酵液灭杀粘虫活性的研究

Insecticidal Activity of Fermentation Broth of Metarhizium anisopliae against Mythimna separata

为了获得有效杀粘虫的生物农药,通过对发酵液杀虫效果的初步测试,从实验室保藏的4株绿僵菌中,筛选出1株效果明显的绿僵菌Ma977.将菌株Ma977的发酵液除去菌体,用其滤液的1、2、4、8倍的稀释液进行室内毒力测试.结果表明:该滤液的原液对粘虫的致死率为100%;随着滤液稀释倍数的增加,致死率逐步降低.对该滤液的有效成分进行液相色谱分析,结果表明,发酵液中含有毒素DtxA、DtxB、DtxB2和DtrxE.

In order to obtain an effective bio-pesticide against armyworm, Metarhizium Ma977 that had significant insecti- cidal activities was selected from 4 Metarhizium strains stored in the laboratory after a primary test of their fermentation broths. After removing Ma977 mycelium and spores, the fermentation broth with 1 time, 2 times, 4 times and 8 times of dilutions were tested. The results showed that the dilutions from the fermentation broth had an insecticidal effect on armyworm. The effect of the dilutions on armyworm was in relation with their concentrations. The non-diluted broth could kill 100% of the armyworm cultured though the mortality rate decreased along with the consistent dilutions. The effective components in the fermentation broth were further analysized by HPLC. The results indicated that the broth contained 4 de- struxins： E, A, B2 and B.