地高辛标记探针原位杂交检测脑膜瘤组织中SV40的表达

Detection of Simian Virus 40 DNA in Meningioma tissues using digoxigenin labeled probe by in situ hybridization

目的：检测猴病毒40（SV40）DNA在人脑膜瘤中的定位表达情况，探讨其在脑膜瘤发生发展过程中的生物学意义。方法：采用地高辛标记探针原位杂交技术。结果：SV40DNA定位于脑膜瘤细胞核，阳性细胞呈弥漫性或局灶性分布；SV40DNA阳性率35．9％（37／103），正常脑组织均未检测出SV40DNA；SV40DNA阳性率与病理分级无关（P＜0．05）。结论：SV40感染与人脑膜瘤病因学密切相关；地高辛标记探针原住杂交技术，是探讨SV40病毒核酸在组织中定位、分布和感染机理的一种新的特异性方法。

To detect the location of simian virus 40 (SV40) and its probable biological function in carciongenesis of meningioma.Methods: Using digoxigenin labeled probe in situ hybridization. Results: Hybridization signals were deected within the nuclei of glioma cells and the positive cells distributed diffusely or focally. SV40 DNA positive rate was 35. 9% (37/103) in meningioma, where as none of the normal brain tissues gave positive results. The correlation between SV40 positive rate and pathological grade was not apparent (P> 0.05). Conclusion:A close relation between SV40 infection and etiology of meningioma was found. The technique of digoxigenin labeled probe in situ hybtidization is a new specific method in studying the location, distribution and infection mechanism of SV40 DNA.