辛伐他汀与环孢素A对大鼠经皮动脉血管成形术术后再狭窄作用的比较

Effects of CsA and simvastatin on the restenosis after percutaneous transluninal angioplasty of abdominal aorta in rats

目的研究环孢素A和辛伐他汀对经皮血管成形术(PTA)后再狭窄作用的差异及两者对炎症因子MCP-1作用的差别。方法选取雄性SD大鼠40只,随机分为对照组(C)、球囊损伤组(BI)、环孢素A组(BI+CsA)和辛伐他汀组(BI+Sim),4组均术前3 d至术后28 d灌胃,术后28 d取材。血管组织做HE染色,光学显微镜下观察病理学改变;ELISA法检测血清中MCP-1含量;RT-PCR法检测血管组织中MCP-1的mRNA表达。结果与C组比较,BI组内膜增殖明显增加,并伴有血浆中MCP-1的含量以及组织中mRNA的表达增加;与BI组比较,药物组内膜增殖明显减少,血浆中MCP-1的含量以及组织中mRNA的表达也明显减少,但仍高于对照组。BI+CsA组与BI+Sim组作用相近(P>0.05)。结论环孢素A和辛伐他汀均能部分减少球囊损伤后再狭窄,两者的抗再狭窄作用可能与两者抑制炎症反应有关,两者作用无明显差别。

Objective To ivestigate the difference between the effects of cyclosporin A（CsA） and simvastatin（Sim） on the restenosis after percutaneous transluninal angioplasty of abdominal aorta in rats.Methods Fourty adult male SD rats were randomly divided into four groups： control group（Group C）,balloon injury group（Group BI）,ballon injury plus cyclosporin A group（Group BI＋CsA）,and ballon injury plus simvastatin group（Group BI＋Sim）.Since 3 days before surgery,CsA and Sim gavage were given to rats in Group BI＋CsA and Group BI＋Sim,respectively,while placebo treatment were given to Group C and Group BI until 28 days after surgery.Then specimens were obtained for evaluation of pathological change,aortic MCP-1 mRNA expression and plasma MCP-1 concentration,with HE staining,real-time PCR and ELISA,respectively.Results Significant hyperplasia of neointima,elevated plasma and aortic MCP-1 expression were revealed in Group BI,when comparing with those in Group C.Although the CsA and Sim treatment partially inhibited the balloon injury-induced neointima hyperplasia and MCP-1 expression,they were elevated when comparing with those in controls.Conclusion CsA and Sim both partially inhibits the restenosis after the balloon injury via depression of inflammation,but there is no significant difference between the two drugs.