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脐带血血清培养脐带血间充质干细胞对脐带血CD34^+细胞的体外扩增作用 被引量:2

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摘要 目的探讨脐带血血清(UCBS)培养的脐带血间充质干细胞(UCB-MSC)对脐带血CD34+细胞体外造血的支持作用。方法采用UCBS培养脐带血贴壁细胞,采用ELISA法检测贴壁细胞培养液中SCF、GM-CSF、IL-6和TPO含量;用流式细胞术分析其免疫表型特征;在成骨细胞和脂肪细胞诱导培养条件下诱导细胞分化,采用脐带血贴壁细胞单独或与细胞因子共培养脐带血CD34+细胞。结果采用UCBS培养脐带血贴壁细胞获得的细胞具有MSC的一般特性,在贴壁细胞培养液中检测到SCF、IL-6和TPO,贴壁细胞和脐带血CD34+细胞培养14 d,联合组第10天和第14天的CD34+细胞的扩增倍数明显高于细胞因子组(P<0.05),联合组第10天明显高于单独UCB-MSCs组(P<0.05),联合组第14天与单独UCB-MSCs组类似,CD34+CD13+亚群细胞比例在联合组均明显高于细胞因子组和单独UCB-MSCs组(P<0.05)。联合组和单独UCB-MSCs组CD34+CD33-亚群细胞比例明显高于细胞因子组(P<0.05)。结论单独应用UCB-MSCs或联合细胞因子可有效地体外扩增脐带血CD34+细胞。
出处 《广东医学》 CAS CSCD 北大核心 2012年第7期919-921,共3页 Guangdong Medical Journal
基金 广东省深圳市龙岗区科技计划项目(编号:YL-2009122)
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共引文献24

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