广州地区献血人群HIV-1 gag基因序列分析

Gene analysis of HIV-1 gag gene in blood donors in Guangzhou

目的了解广州地区献血人群中人类免疫缺陷病毒1型(HIV-1)亚型的流行规律及其与国际参考株的同源性。方法从32例经疾控中心用蛋白印迹法确认为HIV-1阳性的无偿献血者的血浆中提取核酸,用RT-PCR进行gag基因扩增,并对扩增产物进行测序和序列分析。结果 32例标本中有3种HIV-1亚型:14例(43.8%)为CRF01_AE重组亚型,其中12例与国际标准株01_AE.CN.2005.05GX156最接近,平均基因距离为0.043 54;2例与国际标准株01_AE.VN.1998.98VNBG6最接近,平均基因距离为0.056 00;13例(40.6%)为CRF07_BC重组亚型,与国际标准株07_BC.CN.2007.07CNBJ550最接近,平均基因距离为0.026 69;5例(15.6%)为01B型,与国际标准株01B.CN.2008.08CYM003最接近,平均基因距离为0.060 42。结论广州地区献血人群中HIV-1亚型以CRF01_AE和CRF07_BC为主。

Objective To determine the sequence and subtype of HIV-1 in blood donors in Guangzhou and its homogeneity with international reference strains.Methods HIV-1 RNA from HIV positive plasma in 32 blood donors,who was diagnosed as HIV-1 patients with Western Blot,was amplified by RT-PCR.HIV-1 gag gene was sequenced directly from the PCR product.Results There are 3 subtypes of HIV-1 revealed.Among them 14 samples（43.8%） were CRF01_AE,in which 12 were closed to the international standard strain 01_AE.CN.2005.05GX156 with average gene distance of 0.043 54;and 2 were closed to the international standard strain 01_AE.VN.1998.98VNBG6 with average gene distance of 0.056 00.And 13 samples（40.6%） were CRF07_BC,and closed to the international standard strain 07_BC.CN.2007.07CNBJ550 with average gene distance of 0.026 69.The other 5 samples（15.6%） were 01B,and closed to the international standard strain 01B.CN.2008.08CYM003 with average gene distance of 0.060 42.Conclusion The CRF01_AE and CRF07_BC subtypes of HIV-1 accounts the major part in HIV-1 infected blood donors in Guangzhou.