黎族心脑血管疾病患者apo E基因多态性分析

Study on the apo E gene polymorphism in Li population patients with cardiovascular and cerebrovascular disease

目的研究海南黎族人群心脑血管疾病载脂蛋白E(apo E)基因多态性,探讨其与心脑血管疾病之间的关系。方法应用连接酶检测反应(LDR)技术检测150例心脑血管疾病患者(包括高血压50例、脑梗死50例、冠心病50例)和50名健康对照者的apo E基因多态性分布特征,并进行分析。结果高血压组、脑梗死组、冠心病组及正常对照组均以ε3/3基因型频率最高。脑梗死组ε2等位基因频率最低,与高血压组、冠心病组和正常对照组比较差异有统计学意义(P<0.05),高血压组和冠心病组与对照组比较各等位基因差异均无统计学意义(P>0.05)。结论 apo Eε2等位基因降低可能是黎族人群脑梗死发病的重要因素。

Objective To study the apolipoprotein E（apo E） gene polymorphism in Li population patients with cardiovascular and cerebrovascular disease,and investigate the relationship of polymorphism with cardiovascular and cerebrovascular disease.Methods The ligase detection reaction（LDR） technique was used to detect apo E gene polymorphism in 150 patients with cardiovascular and cerebrovascular disease（50 patients with hypertension,50 patients with cerebral infarction and 50 patients with coronary artery disease） and 50 healthy controls.The results were analyzed.Results In the disease group and control group,ε3/3 genotype frequency was the highest.The ε2 allel frequency in cerebral infarction group was lower than those in hypertension group,coronary artery disease group and control group（P0.05）.There was no statistical significance in the allels among the hypertension,coronary artery disease and control groups（P0.05）.Conclusions The apo E ε2 allel may be a major factor to reduce the incidence of cerebral infarction in Li population.