Cx36在癫持续状态大鼠海马神经元的表达

Expression of connexin 36 in status epilepticus rat hippocampal neurons

目的观察连接蛋白36(Cx36)在癫持续状态大鼠海马神经元的表达。方法建立36只氯化锂-匹罗卡品诱导的癫持续状态大鼠模型,随机分为生理盐水组、喹啉组、辛醇组(每组12只),分别予以腹腔注射生理盐水、喹啉、辛醇,通过Racine评分判断大鼠给药前后癫发作情况,利用免疫荧光染色法、Western-blot法检测各组大鼠海马Cx36的表达。结果喹啉组和辛醇组给药前后大鼠行为学评分比较差异有统计学意义(P<0.01);而生理盐水组差异无统计学意义(P>0.05)。喹啉组和辛醇组大鼠海马神经元Cx36的表达明显低于生理盐水组(P<0.01),但2组Cx36的表达量差异无统计学意义(P>0.05)。结论 Cx36在癫发作中起着重要作用,可能成为潜在的治疗靶点。

Objective To observe the expression of connexin 36（Cx36） in status epilepticus in hippocampal neurons. Methods Thirty-six experimental status epilepticus rats induced by lithium chloride-pilocarpine were randomly divided into control group,quinine group,octanol group.Quinine,octanol and saline was administered in vivo.The Racine score was used to decide epileptic seizure before and after administering drug respectively.The expression of Cx36 in hippocampal neurons in each group was determined by using immunofluorescentstaining and Western blotting. Results The Racine score has significant difference before and after administering drug in quinine group and octanol group individually（P0.01）.However,there was no significant difference in saline group（P0.05）.The expression of Cx36 in hippocampal neurons was lower in quinine group and in octanol group than that in control group.However,there was no significant difference in quinine group and octanol group（P0.05）. Conclusion The Cx36 gap junction in neuron may play critical role in seizure activity and may be a potential therapeutic target in epilepsy.