粉防己碱对缺氧和复氧损伤心室肌细胞内Ca^(2+)超载的作用

Effect of tetrandrine on intracelluar Ca 2+ overload of cultured rat cardiomyocytes during hypoxia and reoxygenation

目的 :研究粉防己碱 (Tet)对培养大鼠心室肌细胞缺氧和复氧损伤时细胞内 Ca2 + 超载的作用。方法 :采用荧光探针 Fura- 2 / AM结合计算机图像处理技术测定单个心室肌细胞内 Ca2 + 浓度。结果 :对照组心室肌细胞在缺氧和复氧过程中出现 2次细胞内 Ca2 + 浓度明显上升 ,维拉帕米 (Ver)处理组 (10μmol/ L )心室肌细胞出现 2次细胞内 Ca2 + 浓度轻度上升 ;Tet处理组 (30 0μmol/ L )心室肌细胞未出现细胞内 Ca2 + 浓度上升。两处理组分别与对照组比较均有极显著性差异 (P<0 .0 1) ,两处理组间比较亦有显著性差异 (P<0 .0 5 )。结论 :Tet对缺氧和复氧损伤心室肌细胞内 Ca2 +

Objective:To study the effect of tetrandrine (Tet) on intracelluar Ca 2+ overload of cultured rat cardiomyocytes.Method:Fluorescent probes combined with digital image processing technique were used to measure free Ca 2+ concentration in a cardiomyocyte.Result:Free Ca 2+ concentration in cardiomyocytes rose obviously during hypoxia and reoxygenation in control group,while it rose slightly in verapamil group (verapamil concentration 10 μmol/L),and didn′t change in Tet group (Tet concentration 300 μmol/L).Conclusion:Tet could inhibit Ca 2+ overload of cardiomyocytes remarkably during hypoxia and reoxygenation.