菊米提取液对人胃癌细胞AGS增殖和凋亡的影响

Effect of Jumi Extration on Proliferation and Apoptosis of Human Gastric Carcinoma Cell Line AGS

目的观察菊米提取液对人胃癌细胞株AGS细胞增殖和凋亡的影响。方法 AGS细胞与0.001、0.002、0.005、0.010mg/ml的菊米提取液共同孵育48h后,采用噻唑蓝(MTT)比色法检测细胞增殖抑制率,流式细胞术分析细胞凋亡情况,分光光度法检测caspase-3/7酶及caspase-6酶活性。结果不同浓度(0.001～0.010mg/ml)的菊米提取液对AGS细胞的生长具有明显的抑制作用,呈现出一定的剂量依赖性;含菊米提取液0.002、0.005、0.010mg/ml的药物组细胞凋亡发生率分别为(11.03±2.01)%、(15.53±1.96)%及(23.77±1.65)%,不含药的对照组细胞凋亡发生率为(1.77±0.60)%;与对照组相比,除最低浓度(0.001mg/ml)药物组以外,其余药物组(0.002、0.005、0.010mg/ml)caspase-3/7酶及caspase-6酶活性有显著增强。结论菊米提取液在体外可呈浓度依赖性抑制人胃癌细胞株AGS的增殖并通过caspase-3、6、7通路促进其凋亡。

Objective To explore the effect of Jumi extract on proliferation and apoptosis of human gastric carcinoma cell line AGS. Methods AGS cells and culure media containing Jumi extract （0. 001,0. 002,0. 005,0.01 mg/ml） were co - incubated for 48h. Cell proliferation rate, cell apoptosis, caspase -3/7 and caspase -6 activity were determined by methyl thiazolyl tetrazolium （MTT） colorimet- ric assay, flow cytometry analysis and spectrophotometric detection, respectively. Results The proliferation of AGS cells was significantly inhibited by Jumi extract （0. 001 -O. 01mg/ml） in a dose - dependent manner. Apoptosis rates in the cells treated with Jumi extract （ 0. 002,0. 005,0.01 mg/ml） were （ 11.03 ± 2.01 ） %, （ 15.53 ± 1.96 ） %, and （ 23.77 ± 1.65 ） % respectively, which were higher than that in the control group （ 1.77 ± 0.60） %. Compared with the control group, except lower Jumi treated group （0. 001 mg/ml） , caspase - 3/7 and caspase -6 activity were significantly increased in all Jumi groups. Conclusion The Jumi extract can inhibit the proliferation of gastric carcinoma cell line AGS in vitro in a dose - dependent manner and promote cell apoptosis through caspase - 3, caspase - 7 and caspase - 6 pathway.