CXCR4和MMP-9在胃癌组织中表达及其与淋巴结转移的关系

Correlation of CXCR4 and MMP-9 Expressions with Lymph Node Metastasis in Gastric Cancer

目的探讨胃癌组织中CXCR4和MMP-9的表达与淋巴转移的关系及两者的相关性。方法 S-P免疫组化法及反转录-聚合酶链反应(RT-PCR)检测CXCR4和MMP-9在30例无淋巴转移与46例有淋巴转移人胃癌组织中的表达水平。结果 CXCR4蛋白在胃癌组织中的阳性表达率为55.26%,其中淋巴结转移组阳性表达率分别为73.91%,无淋巴转移组阳性表达率为36.37%,差异有统计学意义(P=0.000)。MMP-9蛋白在胃癌组织中阳性表达率(67.11%),其中淋巴转移组阳性表达率为80.43%,无淋巴结转移者和46.67%,差异有统计学意义(P=0.002)。CXCR4和MMP-9蛋白及mRNA在胃癌中的阳性表达随淋巴结转移增加而增加,且CXCR4阳性表达与VEGF-C阳性表达呈显著正相关(r=0.498,P<0.05)。结论胃癌CXCR4的表达和MMP-9的表达与胃癌淋巴结转移呈正相关,而且两者之间存在一定相关性,联合可能对预测胃癌患者淋巴结转移及判断预后有重要临床价值。

Objective To investigate the correlation of CXCR4 and MMP - 9 expressions with lymph node metastasis in gastric cancer. Methods A total of 30 cases of gastric cancer without lymphatic metastasis and 46 cases of gastric cancer with lymphatic metastasis were enrolled in this study. [mmunohistochemical staining and RT - PCR were performed to detect the expression of CXCR4 and MMP - 9 in gastric： cancer. Results The total positive rate of CXCR4 expression in these samples was 55.26%. It was higher in these cases with lymph node metastasis than that of patients without lymph node metastasis （73.91% vs 36.37% , P = 0. 000）. Meanwhile, the total positive rate of MMP- 9 expression in these samples was 67.11%. And the positive rate with lymph node metastasis was higher than that of patients without lymph node metastasis （ 80.43 % vs 46.67 % , P = 0. 002）. Furthermore, the more lymphatic metastasis, the higher positive expression of CXCR4 and MMP - 9 it was. The expression level of CXCR4 and MMP - 9 were consistent with each other （ r = 0. 406, P 〈 0.01 ）. Conclusion Expressions of CXCR4 and MMP - 9 are closely related to the lymphatic metastasis of gastric cancer, which may provide an evidence for diagnosis and assessment of prognosis in gastric cancer.