摘要
目的探讨核转录因子过氧化物酶体增殖物激活受体-γ(peroxisome proliferator-activated receptor gamma,PPAR-γ)在正常皮肤成纤维细胞和增生性瘢痕成纤维细胞中的表达及其意义。方法原代细胞培养正常皮肤成纤维细胞和增生性瘢痕成纤维细胞,应用实时定量聚合酶链式反应技术(real-time Q-PCR)检测细胞中PPAR-γm RNA的表达;Western blotting技术检测细胞中PPAR-γ蛋白的表达。结果 Real-time Q-PCR结果显示PPAR-γmRNA在正常皮肤成纤维细胞和增生性瘢痕成纤维细胞中均有表达,正常皮肤成纤维细胞中PPAR-γmRNA的表达量明显高于增生性瘢痕成纤维细胞中PPAR-γmRNA的表达量,两者之间的差异有显著性意义(P<0.05);Western blotting结果显示PPAR-γ蛋白在正常皮肤成纤维细胞和增生性瘢痕成纤维细胞中均有表达,增生性瘢痕成纤维细胞中PPAR-γ蛋白明显低于正常皮肤成纤维细胞,两者之间的差异有显著性意义(P<0.05)。结论 PPAR-γ在增生性瘢痕成纤维细胞中表达下降,提示其可能参与了增生性瘢痕的形成过程,调控PPAR-γ有望成为治疗增生性瘢痕的新靶点。
Objective To investigate the expression of peroxisome proliferator - activated receptor gamma （PPAR -γ） in human normal dermal fibroblasts and hypertrophic scar fibroblasts. Methods Human normal dermal fibroblasts and hypertrophic scar fibroblasts were cultured and the expression of PPAR -γ mRNA and protein were detected by real - time Q - PCR and Western blot, respectively. Results The expression of PPAR -γ mRNA and protein were both expressed in human normal dermal fibroblasts and hypertrophic scar fibroblasts, but were lower in hypertrophic scar fibroblasts than in normal dermal fibroblasts, and the differences were significance（ both P 〈 0.05 ）. Conclusion The expression of PPAR —γ, was lower in hypertrophic scar fibroblasts than in normal dermal fibroblasts. It may involved in the formation of hypertrophic scar. Activating of PPAR —γ may be a new target for the treatment of hypertrophic scar.
出处
《医学研究杂志》
2012年第4期145-148,共4页
Journal of Medical Research
