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乳源短乳杆菌M8 S-层蛋白的提纯及其生物学特征分析 被引量：4

Purification and Biological Characterization of S-Layer Proteins in Lactobacillus brevis M8, Isolated from Fresh Milk
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摘要 通过利用原子力显微镜(AFM)来观察凝胶过滤层析法提纯短乳杆菌M8菌株的S-层蛋白的表面形貌,同时探讨S-层蛋白的再生特性及黏附特性。结果表明:凝胶过滤层析法能够获得纯度较高的S-层蛋白;该蛋白在纯水中可自我组装成纳米级"团簇"结构;去除S-层蛋白的菌体细胞仍然具有生命活性,适当培养后可重新表达该蛋白;短乳杆菌M8可黏附到Caco-2细胞上,其S-层蛋白介导此过程。 The aim of this study was to purify S-layer proteins from Lactobacillus brevis M8, isolated from fresh milk, by Sephadex G-75 column chromatography and observe their surface morphology under atomic force microscope （AFM）. Also, regeneration and adhesion characteristics were explored. Highly pure S-layer proteins were obtained by Sephadex G-75 column chromatography, which could form nano-cluster by self-assemblage. Lactobacillus brevis M8 cells without S-layer proteins could re-express the proteins after re-incubation. Lactobacillus brevis M8 could adhere to Caco-2 cells, which was mediated by the S-layer proteins.
作者 肖荣 范郁冰 张丽丽 李宗军
机构地区 食品科学与生物技术湖南省重点实验室 湖南省发酵食品工程技术研究中心 国家植物功能成分利用工程技术研究中心
出处 《食品科学》 EI CAS CSCD 北大核心 2012年第7期172-175,共4页 Food Science
基金 国家"863"计划项目(2007AA10Z347) 湖南省研究生科研创新项目(54040108005/12254)
关键词 短乳杆菌M8 S-层蛋白 AFM形貌 黏附 Lactobacillus brevis M8 S-layer proteins atomic force microscope （AFM） images adhesion
分类号 Q933.31 [生物学—微生物学]
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参考文献16

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共引文献9

同被引文献62

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引证文献4

二级引证文献11

食品科学
2012年 第7期

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