浒苔对营养性肥胖小鼠减肥功能的研究

Effect of Enteromorpha on Body Weight Loss in Nutritionally Obese Mice

目的:研究浒苔对营养性肥胖小鼠的减肥作用。方法:用营养饲料建立肥胖模型,造模成功小鼠分为模型组,浒苔高、中、低剂量组和阳性对照组。模型组小鼠喂以基础饲料并灌胃清水,浒苔高、中、低剂量组分别喂以添加质量分数30%、20%、10%浒苔的基础饲料并灌胃清水,阳性对照组喂以基础饲料并灌胃50mg/(100g.d)(以体质量计)的左旋肉碱。49d后眼球取血,测定小鼠体质量、Lee,s指数、脂肪系数及血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量和丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性。结果:高、中、低剂量浒苔及左旋肉碱均能有效降低雌性与雄性营养性肥胖小鼠体质量、Lee,s指数、脂肪系数及血清TC、TG、LDL-C含量和ALT、AST活性,升高HDL-C含量和HDL-C含量/T C含量。结论:浒苔具有良好的减肥功能,其机理可能是抑制脂肪生成、调节血脂、保护肝功能。

Objective： To explore the body weight-reducing effect of Enteromorpha in nutritionally obese mice. Methods： A nutritionally obese mouse model was established by oral administration of a nutritional diet. The mice were divided into five groups including model group （T-cont group）, Enteromorpha groups at high, medium and low dosages （T-high group, T-med group and T-low group） and positive control group （P-cont group）. The mice from T-cont group were fed a basal diet. The mice from T-high group, T-med group and T-low group were fed a basal diet with the addition of 30%, 20% and 10% Enteromorpha, respectively. The mice from P-cont group were fed a basal diet supplemented with L-carnitine at 50 mg/（100 g· d）. After administration for 49 days, blood was drawn from mouse eyeballs. The contents of serum TC, TG, LDL-C and HDL-C, the activities of serum ALT and AST, body weight, Lee＇s index and fat coefficient were evaluated. Results： Enteromorpha at high, medium and low dosages and L-carnitine could significantly decrease body weight, Lee＇s index and fat coefficient, the contents of serum TC, TG and LDL-C and the activities of serum ALT and AST, and increase HDL-C content and I-IDL-C/TC ratio in male and female nutritionally obese mice. Conclusion： Enteromorpha has obvious weight loss function. Its possible mechanisms may be involved in the suppression of fat accumulation, regulation of blood lipid and protection of liver function.