薛荔籽果胶对去势雌性大鼠矿物质吸收的影响

Effect of Pectin from Ficus pumila L. Seeds on Mineral Absorption in Ovariectomized Rats

目的:探讨薛荔籽果胶对去势雌性大鼠矿物质吸收的影响。方法:将30只SD成年雌鼠随机分为5组。4组大鼠做双侧卵巢切除手术,一周后分为空白组和薛荔籽果胶高、中、低剂量组;用伪切除同龄大鼠作对照(Sham组)。喂养4周后解剖,测定盲肠内容物pH值、短链脂肪酸、骨钙、骨磷含量及矿物质元素的表观吸收等指标。结果:去势雌性大鼠对钙、镁、磷、锌和铁元素的表观吸收率及骨钙和骨磷含量显著低于正常大鼠(P<0.05)。薛荔籽果胶能显著增加去势雌性大鼠短链脂肪酸含量和盲肠面积(P<0.05),降低盲肠pH值,并且在一定程度上抑制钙、镁、磷、锌和铁元素的表观吸收率。结论:薛荔籽果胶能降低去势雌性大鼠矿物质的表观吸收率。

Objective： To investigate the effect of pectin from Ficus pumila L. seeds on mineral absorption in ovariectomized rats. Methods： Thirty SD female rats were divided into five groups. The mice in four of them were double ovariectomized and one week later assigned as model control group and high, medium and low dose groups ofFicuspumila L. pectin. The mice in the fifth group were sham-ovariectomized as sham control. All rats were sacrificed after 4 weeks of feeding to assay pH values and short-chain fatty acids in cecum contents, calcium and phosphorus in bone, and apparent absorption rate of minerals. Results： Compared to the sham control group, the apparent absorption rates of calcium, magnesium, phosphorus, zinc and iron, calcium and phosphorus in bone significantly decreased in OVX rats （P〈0.05）. Pectin from Ficus pumila L. seeds drastically increased the concentration of short-chain fatty acids in cecum content and the area of cecum, decreased the pH of cecum contents, and inhibited the absorption of calcium, magnesium, phosphorus, zinc and iron in ovariectomized rats. Conclusions： Ficus pumila L. seed pectin had a negative effect on mineral absorption in ovariectomized rats.