不同颈椎内固定融合术式实验兔模型的建立

Rabbit model for different internal fixation and fusion methods of cervical spine

目的建立兔颈椎不同术式内固定融合模型。方法将60只新西兰白兔随机分3组,每组20只。A组行颈椎前路椎体间融合内固定术,B组行颈椎后路椎板棘突间融合内固定术,C组行颈椎前、后路联合融合内固定术。造模后1个月和3个月,每组10只行安乐死取材,分别摄X线片、作大体及组织形态学观察。结果随着时间的推移,X线片可见A、C组固定节段椎间隙逐渐融合、消失,B、C组固定节段椎板棘突融合。术后1个月标本见骨小梁、胶原纤维排列有序、规则,骨外膜侧尚有部分纤维组织,表明其有骨化及成骨现象;术后3个月标本见类似于正常椎体骨或椎板骨,胶原纤维折光性强,方向有序。结论建立兔颈椎内固定融合模型方法简单、易行、可靠,为脊柱疾病治疗方法研究提供良好的平台。

Objective To establish animal model of different operation methods for internal fixation and fusion of cervical spine.Methods Sixty New Zealand white rabbits were randomly divided into three model groups.The first model group（A group） were undergone anterior cervical spine internal fixation and fusion.The second model group（B group） were undergone posterior cervical spine internal fixation and fusion.The third model group（C group） were undergone anterior and posterior cervical spine internal fixation and fusion at the same time.After operation,the rabbits were examined respectively by X-ray at first,third month and then ten rabbits in every group were sacrificed for pathological observation.Results By the time,three model groups had abnormal X-ray signs in the segment of internal fixation and fusion： In A,C groups,the intervertebral space disappeared.In B,C groups,the vertebral plate and spinous process space were disappeared.By the end of first month,trabeculae of bone and collagen fiber were in order and regulation.The periosteum side had some fibrous tissue.These may manifestation there had ossification phenomenon.After three months,the ossification was similar to body of vertebra or vertebral plate.The refraction of collagen fibers was powerfully and arrange an order direction.Conclusions The models for internal fixation and fusion of cervical spine were simple,easy and reliability.It can provide a satisfactory model to study and research healing of spinal column disease.