米非司酮对早孕外周血和蜕膜组织自然杀伤细胞亚群的影响

The effect of mifepristone on natural killer subsets of peripheral blood and decidua in early pregnancy

目的和方法 :采用流式细胞仪检测淋巴细胞亚群法 ,探讨米非司酮对早孕外周血和蜕膜NK细胞亚群的影响。结果 :早孕和米非司酮处理组外周血的NK细胞各亚群的百分率近似 ;米非司酮处理组血清E2和P4 均稍高于早孕组。而早孕蜕膜组的三个NK细胞亚群的百分率均明显高于米非司酮处理组。米非司酮处理组的蜕膜以CD5 6 +NK细胞为主要淋巴细胞亚群 ,而外周血则以CD5 6 +CD16 +和CD16 +NK细胞为主。结论 :米非司酮主要作用于早孕母胎界面 ,阻断蜕膜CD5 6 +NK细胞的增殖和分化 。

AIM: To investigate the effect of mifepristone on natural killer(NK) subpopulations of peripheral blood and decidua in early pregnancy. METHODS: Flow cytometry was used to detect the expression of CD56 and CD16 on lymphocytes of decidua and peripheral blood in early pregnancy, mifepristone-treated pregnancy. RESULTS: The percentages of different natural killer subsets in peripheral blood between early pregnancy and mifepristone-treated pregnancy were almost identical. The serum levels of estradiol and progesterone in mifepristone-treated pregnancy were slightly higher than in early pregnancy. The percentage of decidual CD56+NK cell in early pregnancy was significantly higher than in mifepristone-treated pregnancy. The CD56+NK cells were predominant lymphocyte population of decidua in mifepristone-treated pregnancy, but in which the CD56+CD16+ and CD16+NK cells were major lymphocyte subpopulations of peripheral blood. CONCLUSION: Mifepristone acted principally on feto-maternal interface, it blocked the proliferation and differentiation of decidual CD56+NK cells and induced embryo immune rejection.