双歧杆菌的全肽聚糖对裸鼠腹腔巨噬细胞产生IL-6、IL-12及一氧化氮的影响

Influence of whole peptidoglycan of bifidobacterium on interleukin 6, interleukin 12 and nitric oxide produced by peritoneal macrophages of nude mice

目的 :探讨分叉双歧杆菌 (B bifidum)的全肽聚糖对巨噬细胞功能的调节作用。方法 :首先用全肽聚糖注射于裸鼠腹腔 ,以ELISA法测定裸鼠腹腔巨噬细胞产生的IL - 6和IL - 12含量 ,同时用Griess试剂检测一氧化氮 (NO)的水平。结果 :全肽聚糖注射组裸鼠腹腔巨噬细胞产生的IL - 6、IL - 12及NO含量分别为732 5 4± 190 30 (pg/mL)、816 37± 96 40 (pg/mL)和 48 90± 6 5 1(μmol/L) ;对照组分别为 30 3 78± 171 75 (pg/mL)、5 10 2 7± 12 3 46 (pg/mL)以及 30 6 7± 12 83(μmol/L) ,三者比较均具有显著差异 (P <0 0 1)。 结论 :分叉双歧杆菌的全肽聚糖能激活巨噬细胞 ,使之分泌多量的IL - 6、IL - 12及NO ,这些细胞毒性效应分子介导了全肽聚糖的多种重要生理功能。

AIM: To deplore the adjustion of whole peptidoglycan of bifidobacterium bifidum to functions of macrophages.METHODS:Levels of interleukin 6, interleukin 12 produced by peritoneal macrophages of nude mice was detected by ELISA method, and the content of nitric oxide (NO) was detected by using Griess reagent, after whole peptidoglycan were injected into nude mice peritoneally. RESULTS:The content of interleukin 6, interleukin 12 and NO secreted from peritoneal macrophages of nude mice in the whole peptidoglycan injection group was 732.54±190.30(pg/mL)、816.37±96.40(pg/mL) and 48.90±6.51(μmol/L) respectively.It was 303.78±171.75(pg/mL), 510.27±123.46(pg/mL) and 30.67±12.83(μmol/L) respectively in the control group. In view of the content of interleukin 6, interleukin 12 and NO, there existed significant difference when whole peptidoglycan injection group was compared with control group( P< 0.01).CONCLUSION:The result suggested that whole peptidoglycan of bifidobacterium bifidum could activate macrophages to secrete large amount of interleukin 6, interleukin 12 and NO. These cytotoxicity effectors mediated many kinds of important physiological functions of bifidobacterium.