脂多糖、白细胞介素-6、肿瘤坏死因子α对乳鼠星形胶质细胞表达组织因子的影响

Effects of lipopolysaccharide,IL-6 and TNFα on the tissue factor expression of astrocytes

目的 :观察脂多糖、白介素 - 6和肿瘤坏死因子α对大鼠星形胶质细胞组织因子活性表达的影响。方法 :培养Wistar乳鼠星形胶质细胞 ,细胞用抗神经胶质纤维酸性蛋白单克隆抗体确认。细胞冻融液组织因子活性检测采用一期血浆复钙时间法。结果 :脂多糖、白介素 - 6和肿瘤坏死因子α组星形胶质细胞表达组织因子活性明显高于对照组 (P <0 .0 5 )。脂多糖、白介素 - 6和肿瘤坏死因子α与三氟吡啦嗪 (TFP)或 1- (5 -异喹啉磺胺 ) - 3 -甲基哌嗪 (H7)联合运用 ,则它们的刺激作用受到明显抑制。结论 :脂多糖、白介素 - 6、肿瘤坏死因子能刺激星形胶质细胞表达组织因子活性 ,其机制可能与钙 /钙调素和蛋白激酶C途径有关。

AIM:To study the effects of lipopolysaccharide(LPS), interleukin-6(IL-6)and tumor necrosis factor α (TNFα) on tissue factor(TF) expression of astrocytes. METHODS:Astrocytes were identified with anti-glial fibrillary acidic protein antibody. The TF activity of cell lysate was measured with one stage clotting assay. RESULTS:TF activity of astrocytes of LPS,IL-6,TNFα groups were obviously higher than that of the control group( P <0.05); While LPS,IL-6 and TNFα were combined with trifluoperazine or H 7, their inductive effects were inhibited. CONCLUSION:LPS,IL-6 and TNFα promoted the TF expression of astrocytes and its mechanisms may connected with Calcium/Camodulin and protein kinase C pathway.