青蛤(Cyclina sinensis)生长性状相关分子标记的AFLP分析

AFLP ANALYSIS OF MOLECULAR MARKERS RELATED TO GROWTH TRAITS IN CYCLINA SINENSIS

采用AFLP技术和单因素方差分析方法,进行了青蛤主要生长性状相关分子标记筛选研究。结果表明,检测的18个与壳长相关的基因位点,其贡献率在6.46%—18.32%之间,其中E32M51-8、E33M51-38对壳长的贡献率较高,分别为18.32%和15.68%。检测的23个与壳宽相关的基因位点,其贡献率在6.75%—20.93%之间,其中E32M51-8、E33M48-19对壳宽的贡献率较高,分别为20.93%和17.34%。检测的22个与壳高相关的基因位点,其贡献率在6.54%—15.91%之间,其中E32M51-17对壳高的贡献率最高,为15.91%。检测的8个与活体重相关的基因位点,其贡献率在6.65%—17.83%之间,其中E33M48-10对活体重的贡献率最高,为17.83%。以上结果说明青蛤生长性状的遗传基础复杂,通过筛选与其相关显著的分子标记,在育种中开展分子标记选择是可行的。

Abstract In this paper, 60 mixed breeding offsprings from two natural populations of Cyclina sinensis were used to screen molecular markers related to shell length, shell width, shell height and body weight under the method of AFLP and one-factor analysis of variance. The results showed： of detected 18 gene loci related to shell length, the contribution rates were between 6.46% and 18.32%, and the contribution rates of E32M51-8, E33M51-38 were higher, respectively 18.32% and 15.68%. The contribution rates of detected 23 gene loci associated with shell width were between 6.75% and 20.93%, and those of E32M51-8, E33M48-19 were higher, respectively 20.93% and 17.34%. For shell height, 22 associated gene loci were detected, their contribution rates were between 6.54% and 15.91%, and the E32M51-17 locus had the highest contribution rate. Eight gene loci related to body weight were analyzed, the highest contribution rate （locus E33M48-10） was 17.83%, and lowest value was 6.65%.