来曲唑与三苯氧胺对人卵巢癌顺铂耐药细胞株SKOV3/DDP生长及耐药的影响

Effects of letrozole and tamoxifen on the growth and drug resistance of cisplatin-resistant human ovarian cancer cell line SKOV3/DDP

目的:研究来曲唑(letrozole,LTZ)与三苯氧胺(tamoxifen,TAM)对人卵巢癌顺铂耐药细胞株SKOV3/DDP生长及耐药的影响。方法:采用四甲基偶氮唑蓝(MTT)法,比较不同浓度的LTZ、TAM、LTZ+TAM对SKOV3/DDP细胞生长及对顺铂(cisplatin,DDP)耐药的逆转作用。结果:①不同浓度(1×10-9mol/L～1×10-4mol/L)LTZ或低浓度(1×10-9mol/L～1×10-7mol/L)TAM对SKOV3/DDP细胞生长无抑制,不能逆转DDP引发的耐药。高浓度(1×10-6mol/L～1×10-4mol/L)TAM能抑制SKOV3/DDP细胞的生长且能逆转DDP引发的耐药,与对照组比较,差异有统计学意义(P<0.05)。②1×10-6mol/L TAM与各浓度(1×10-9mol/L～1×10-4mol/L)LTZ联合用药,对该细胞的生长抑制作用及逆转DDP引发的耐药较单用1×10-6mol/L TAM无明显增强,差异无统计学意义(P>0.05);(1×10-5mol/L～1×10-4mol/L)TAM与各浓度(1×10-9 mol/L～1×10-4 mol/L)LTZ联合用药,在LTZ为(1×10-6 mol/L～1×10-4 mol/L)时,两药联合对该细胞的生长抑制作用与单用TAM(1×10-5mol/L～1×10-4mol/L)及组间相比差异有统计学意义(P<0.01);在LTZ为(1×10-5mol/L～1×10-4mol/L)时,两药联合逆转DDP引发的耐药与单用TAM(1×10-5mol/L～1×10-4mol/L)逆转及组间相比,差异有统计学意义(P<0.01)。结论:高浓度TAM可以逆转SKOV3/DDP细胞对DDP的耐药性;高浓度LTZ与TAM联合可使TAM逆转SK-OV3/DDP细胞对DDP耐药的作用增强。

Objective：To study the effects of letrozole and tamoxifen on the growth and drug resistance of cisplatin-resistant human ovarian cancer cell line SKOV3/DDP.Methods：MTT method was used to compare the reversal effects of different concentrations of letrozole,tamoxifen,and letrozole plus tamoxifen on the growth and drug resistance of cisplatin-resistant human ovarian cancer cell line SKOV3/DDP.Results：Different concentrations（1×10^-9 mol/L-1×10^-4 mol/L） of letrozole and low concentration tamoxifen had no impact on the growth of SKOV3/DDP cells,which couldn＇t reverse drug resistance to DDP.High concentration（1×10^-6 mol/L-1×10^-4 mol/L） tamoxifen could repress the growth of SKOV3/DDP cells and reverse drug resistance to DDP,compared with control group,there was significant difference（P〈0.05）.There was no significant difference in the inhibiting effect on the growth of SKOV3/DDP cells and the reversal effect on drug resistance to DDP between combined application of 1×10^-6mol/L tamoxifen and different concentrations（1×10^-9 mol/L-1×10^-4 mol/L） of letrozole and simple application of 1×10^-6mol/L tamoxifen（P〈0.05）.There was significant difference in the inhibiting effect on the growth of SKOV3/DDP cells between combined application of letrozole（1×10^-6 mol/L-1×10^-4 mol/L） and tamoxifen（1×10^-5 mol/L-1×10^-4 mol/L） and simple application of tamoxifen（1×10^-5 mol/L-1×10^-4 mol/L）（P〈0.01）.There was significant difference in the reversal effect on drug resistance to DDP between combined application of letrozole（1×10^-5 mol/L-1×10^-4 mol/L） and tamoxifen（1×10^-5 mol/L-1×10^-4 mol/L） and simple application of tamoxifen（1×10^-5 mol/L-1×10^-4 mol/L）（P〈0.01）.Conclusion：High concentration tamoxifen can reverse the drug resistance of SKOV3/DDP cells to DDP,high concentration letrozole combined with tamoxifen can enhance the reversal effect of tamoxifen on drug resistance to DDP.