长期使用葛根素对去卵巢大鼠阴道壁结构的影响

Effect of long-term application of puerarin on vaginal wall structure of ovariectomized rats

目的:观察长期使用葛根素对去卵巢大鼠阴道壁结构的影响,为中西药葛根素与雌激素组合治疗Ⅰ型骨质疏松症提供实验依据。方法:5月龄健康雌性大白鼠40只,分成5个实验组(n=8只),除假手术组外均建立去卵巢动物模型。①假手术组(Sham);②去卵巢模型组(OVX);③葛根素-50组(Pr-50),皮下注射葛根素50 mg/kg,1次/天;④雌二醇-200组(E2-200),皮下注射雌二醇200μg/kg,2次/周;⑤葛根素-25+雌二醇-100组(Pr-25+E2-100),皮下注射葛根素25 mg/kg,1次/天和雌二醇100μg/kg,2次/周。在第20周,取大鼠血清和阴道壁,观察血清雌二醇(E2)水平和阴道壁结构的变化。结果:OVX组E2水平、阴道上皮及肌层厚度明显低于其他各组(P﹤0.05);E2-200组E2水平、阴道上皮及肌层厚度明显高于其他各组(P﹤0.05);Pr-25+E2-100组E2水平较Pr-50组高(P﹤0.05),但两组间阴道上皮及肌层厚度差异无统计学意义(P﹥0.05)。结论:较大剂量雌二醇可明显促进去卵巢大鼠阴道上皮和肌层增生;小剂量的雌二醇与葛根素联合、较大剂量的葛根素对去卵巢大鼠阴道上皮和肌层增生作用不明显。

Objective：To observe the effect of long-term application of puerarin on vaginal wall structure of ovariectomized rats,provide a experimental basis for treating type Ⅰ osteoporosis by combination therapy of puerarin and estrogen.Methods：Forty healthy female rats of five months old were randomly divided into five experimental groups,eight rats in each group：sham operation group,ovariectomized model group,puerarin-50 group（the rats were treated with subcutaneous injection of puerarin（50 mg/kg）,once a day）,estradiol-200 group（the rats were treated with subcutaneous injection of estradiol（200 μg/kg）,twice a week）,puerarin-25 plus estradiol-100 group（the rats were treated with subcutaneous injection of puerarin（25 mg/kg,once a day） plus estradiol（100 μg/kg,twice a week））,except sham operation group,ovariectomized rat models were established in the other four groups.The serum and vaginal wall samples were obtained on the twentieth weeks,the changes of serum estradiol levels and vaginal wall structures in the five groups were observed.Results：The serum estradiol level,vaginal epithelial thickness and myometrial thickness in ovariectomized model group were significantly lower than those in the other four groups（P〈0.05）.The serum estradiol level,vaginal epithelial thickness and myometrial thickness in estradiol-200 group were significantly higher than those in the other four groups（P〈0.05）.The serum estradiol level in puerarin-25 plus estradiol-100 group was significantly higher than that in puerarin-50 group（P〈0.05）,but there was no significant difference in the vaginal epithelial thickness and myometrial thickness between the two groups（P〈0.05）.Conclusion：High-dose estradiol can promote hyperplasia of vaginal epithelium and myometrium among ovariectomized rats obviously;the impacts of low-dose estradiol combined with puerarin,high-dose puerarin on hyperplasia of vaginal epithelium and myometrium among ovariectomized rats are not obvious.