Patatin启动子驱动的乙肝表面抗原基因在马铃薯中的表达

Expression of the HBsAg Gene Under the Control of a Patatin Promoter in Potato (Solanum tuberosum)

按常规分子克隆方法将马铃薯中高表达的patatin启动子和乙肝表面抗原基因分别插入双元质粒pBI101成为植物表达载体pPHG9,由前者驱动后者表达;通过农杆菌转化法将乙肝表面抗原基因导入马铃薯并获得再生植株;用ELISA方法检测经PCR鉴定的转基因植株中乙肝表面抗原的含量。

A plant expression vector pPHG9 was constructed through the insertion of a fusion gene into a binary vector pBI101 in which the HBsAg gene was placed under the control of a high level expressed patatin promoter. The HBsAg gene was then introduced into potato via Agrobacterium mediated transformation. Regenerated transformed potato plants were confirmed by PCR and ELISA results. The contents of HBsAg protein in transgenic potato plants were determined by ELISA, varying from 100 174ng/mg soluble protein.