瑞克纤孔菌发酵条件优化及菌丝化学成分定性分析

Fermentation optimization of Inonotus rickii and qualitative analysis of the chemical ingredients in its mycelia

研究了瑞克纤孔菌液体发酵适宜的碳源、氮源、温度和pH值,并定性分析了菌丝体的主要化学成分.结果表明,玉米淀粉、甘露醇和葡萄糖为适宜碳源,以玉米淀粉为最适;硝酸钠等无机氮源不适宜该真菌生长,适宜氮源为蛋白胨和酵母粉,以蛋白胨为最适;菌丝生长的适宜pH=6.0～6.5,适宜温度为25～30℃.瑞克纤孔菌菌丝体含有氨基酸、多肽、蛋白质、有机酸、还原糖、多糖和甙、皂甙、甾体和三萜类化合物、内酯、香豆素和挥发油,不含有生物碱、酚类化合物、鞣质、黄酮类化合物、强心甙和蒽醌类物质.

The cultural condition for Inonotus rickii including carbon source,nitrogen source,temperature and pH was studied and the main chemical ingredients in its mycelia were analyzed.The results showed that corn starch,mannite and glucose were the preferential carbon source,the best was corn starch;sodium nitrate did not suit for production of fungus peptone and dusty yeast was the preferential nitrogen source,the best was peptone.The optimal cultural condition for Inonotus rickii was pH=6.0～6.5 and temperature 25～30 ℃.The chemical ingredients in its mycelia were amino acids,pdypeptide,protein,organic acid,reducing sugar,polysaccharide,glucoside,sapogenin,steroids,triterpenoid,lactones,coumarin and naphtha and do not include alkaloid,phenols,tannin,flavone,cardiac glycoside and anthraquinone.