摘要
以4-羧基苯甲醛,N,N-二甲基苯胺为原料合成羧基化隐性孔雀石绿(COOH-LMG),经薄层色谱、红外光谱、紫外光谱、液质联用仪鉴定该羧基化隐性孔雀石绿为目标产物。羧基隐性孔雀石绿经碳二亚胺法与牛血清白蛋白(BSA)、卵清蛋白(OVA)载体蛋白相结合,分别得到免疫原与包被原。通过紫外分光光度计鉴定,人工抗原偶联物的吸收峰较反应前的反应物的吸收峰发生了漂移,证明耦合成功。新西兰大白兔经免疫原5次免疫后,得到针对隐性孔雀石绿的抗体,效价达1∶64000,为LMG的单抗的制备及其免疫学分析方法提供了重要依据。
Carboxy-leucomalachite green（COOH-LMG）was synthesized facilely with N,N-dimethylanilin and 4-Carboxybenzaldehyde as raw materials,and it was identified by the thin layer chromatography（TLC）,infrared spectrometry（IRS）,ultraviolet spectrum（UV）and liquid chromatography-mass spectrometer（LC-MS）.COOH-LMG was conjugated to bovine serum albumin（BSA）,ovalbumin（OVA）to form antigen and immunogen,and they were identified by UV spectra analysis.The antibodies against leucomalachite green（LMG）were obtained from rabbits after five times immunizations.The titer of the polyclonal antibody against the LMG was about 1：64000 by indirect ELISA.It laid the foundation for further gain monoclonal anti-LMG and its immunological assay.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第9期171-174,共4页
Science and Technology of Food Industry
基金
湖北省自然科学基金(2008CDB357)
武汉工业学院研究生创新基金(09cx024)
关键词
隐性孔雀石绿
合成
半抗原
人工抗原
多克隆抗体
leucomalachite green
synthetize
hapten
complete antigens
polyclonal antibody
