鹅胆汁中鹅脱氧胆酸和胆红素提取工艺研究

Study on extraction of chenodeoxycholic acid and bilirubin fromgoose gallbladder

鹅脱氧胆酸(CDCA)和胆红素均是鹅胆中的主要活性成分。将鹅胆通过皂化、中和、分层等步骤,同时从中分离提取出鹅脱氧胆酸和胆红素。通过单因素实验和正交实验确定了皂化温度、皂化时间和NaOH浓度的最佳条件。实验结果表明,当皂化温度为90℃,皂化时间为1h,NaOH浓度为8%时,CDCA的提取量为0.9mg/g,胆红素的提取量为0.46mg/g。

Both chenodeoxycholic acid and bilirubin are the main active components of goose gallbladder.The chenodeoxycholic acid and bilirubin were isolated and extracted from the goose gallbladder in the same time through the steps of saponification,neutralization,delamination and so on.The effect of saponification temperature,time and sodium hydroxide concentration were determined through the single factor and orthogonal tests.The results showed that the optimum conditions were as follows：saponification temperature 90℃,1h,sodium hydroxide concentration 8%.Under these conditions,the yield of chenodeoxycholic acid reached 0.9mg/g,the yield of bilirubin reached 0.46mg/g.