西藏芜菁营养成分测定及提高缺氧耐受性的动物实验研究

Determination on nutritional content of Tibetan turnip(Brassica rapa L.) and experimental study on improvement of mice hypoxia tolerance

芜菁是产自藏区的一种古老作物,是藏民及其爱畜牦牛进入高海拔地区作业的必备食材,栽培历史悠久。以西藏芜菁为原料,参照国标方法测定了其氨基酸组成,维生素和微量元素含量,发现其氨基酸组成种类繁多,微量元素含量丰富,并对其抗缺氧能力进行了研究,分别用低、中、高三个剂量(0.5、1.0、2.0g/kg.d)的芜菁冻干粉连续灌胃ICR小鼠7天,并设置红景天阳性药物对照组和蒸馏水空白对照组,用秒表记录小鼠在缺氧条件下的存活时间,解剖后取肝脏测定蛋白含量和SOD活力。研究结果表明,芜菁粉的高剂量组和阳性对照组能显著改善小鼠的缺氧耐受力,中、高剂量组和阳性对照组的肝脏SOD活力均得到显著提高。同时,各组实验小鼠间的体重和肝体指数均无显著差异。结果表明西藏芜菁能够显著提高实验小鼠的缺氧耐受性和体内抗氧化活性。

Turnip（Brassica rapa L.）was a magic plant in high altitudes,it was used as a kind of food with a long history for Tibetan and yak.We used Tibetan turnip as material,assay the amino acid,mineral element and Vitamins contents in it.The anti-hypoxic effect of turnip frozen-dry powder on ICR mice was studied.To investigate the extension of the mice hypoxia tolerance,three doses（0.5,1.0,2.0g/kg·bw·d）was set.And Rhodiola rosea was taken as a positive control and distilled water was as a blank control,the survival time of mice in hypoxia under ordinary pressure was the evaluating indicator.Weigh the mice liver and assay protein contents and SOD activity.The research indicated that high dose at 2.0g/kg·bw·d of turnip powder and positive control showed a significant extension effect on the mice hypoxia tolerance;mid-and high-doses as well as positive control had a significant promotion on liver SOD activity;and the body weight index and hepatopancreas somatic indices of all tested mice were not of obvious difference.It indicated that Tibetan turnip could improve mice hypoxia tolerance and the antioxidation capacity in vivo of mice.