沙棘果皮渣黄酮毒理学初步评价

Preliminary toxicology evaluation of Hippophae rhamnoidesmarc flavones

以沙棘果皮渣黄酮为受试物,经口染毒针对ICR小鼠做急性毒性实验、小鼠骨髓细胞染色体畸变实验、小鼠骨髓细胞微核实验、小鼠精子畸变实验,对受试物进行急性毒性实验及初步遗传毒性评价。实验结果表明:小鼠经口染毒最大耐受量(MTD)为24g/kg(>15g/kg),受试物的急性毒性分级为无毒级;在MTD以下分设高(8g/kg)、中(4g/kg)、低(2g/kg)三个剂量组考察受试物的遗传毒性,实验结果为小鼠骨髓染色体畸变率、小鼠骨髓细胞微核率以及小鼠精子畸变率均未发现增高,表明受试物不能导致小鼠骨髓细胞染色体畸变,不会诱发小鼠嗜多染红细胞微核产生,对小鼠精子无致畸作用。

The acute toxicity and genetic toxicity of Hippophae rhamnoides were performed by acute oral toxicity test in ICR mice,such as chromosome aberration test of bone marrow cells of mice,the micronucleus test of bone marrow cells of mice and the sperm abnormality text of mice.The result showed that the oral maximum tolerance dose of mice（MTD）was 24g/kg.According to acute toxicity classification criteria,test substance belongs to non-toxic.Under the dose of less than 24g/kg,high dose group（8g/kg）,middle dose group（4g/kg）and low dose group（2g/kg）were set up to check the genetic toxicity of test substance.Result showed that the chromosome aberration rate,the micronucleus rate and the sperm abnormality rate didn＇t increase,what improved that test substance would not cause the aberration of chromosome,increase the micronucleus amount of mice polychromatic erythrocyte and lead to sperm abnormality.