亚硒酸钠对脑胶质瘤细胞生长及线粒体膜电位的影响

Inhibitory Effect of Sodium Selenite on the Growth of Human Glioma Cell and Its Effect on Mitochondrial Membrane Potential

目的:研究亚硒酸钠对人脑胶质瘤细胞生长抑制效应及线粒体膜电位的影响。方法:使用不同浓度的亚硒酸钠(0.004、0.020、0.100及0.500μmol/L)对人脑胶质瘤细胞SHG-44进行侵染,通过四甲基偶氮唑盐(MTT)比色法检测亚硒酸钠对SHG-44增殖的影响,提取细胞线粒体后通过激光扫描共聚焦显微镜下观察SHG-44的荧光强度,分析SHG-44的线粒体膜电位。结果:亚硒酸钠对人脑胶质瘤细胞生长具有明显的抑制作用,且随亚硒酸钠浓度的增加抑制作用逐渐加强,0.100及0.500μmol/L染硒组与对照组相比差异均有统计学意义(P<0.05),0.004及0.020μmol/L染硒组与对照组相比差异无统计学意义(P>0.05);0.100、0.500μmol/L染硒组线粒体膜电位显著降低(P<0.05),0.004及0.020μmol/L组与对照组相比差异无统计学意义(P>0.05)。结论:亚硒酸钠能抑制人脑胶质瘤细胞SHG-44生长,并且降低其线粒体膜电位。

Objective： To investigate the inhibitory effects of sodium selenite on the growth of human glioma cell SHG-44 and its effect on mitochondrial membrane potential.Methods： SHG-44 cells were treated with different concentrations（0.004 μmol/L,0.020 μmol/L,0.100 μmol/L,0.500 μmol/L）of sodium selenite.The effect of sodium selenite on proliferation of SHG-44 cells was detected by MTT assay.The mitochondria was extracted,and fluorescence intensity of SHG-44 cells were detected under laser scanning confocal microscopy,and mitochondrial membrane potential of SHG-44 cells was analyzed.Results： Sodium selenite significantly suppressed the growth of SHG-44 cells.The cell growth rate decreased with the concertration increase of sodium selenite.Statistical differences were found between 0.100 μmol/L,0.500 μmol/L groups and control group（P0.05）,but not between 0.004 μmol/L,0.020 μmol/L groups and control group（P0.05）.Mitochondrial membrane potential was decreased significantly in 0.100 and 0.500 μmol/L groups（P0.05）,but not in 0.004 μmol/L and 0.020 μmol/L groups（P0.05）.Conclusions： Sodium selenite can suppress the SHG-44 cell growth,and down-regulate the mitochondrial membrane potential.