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宁夏腹泻病人群携带NDM-1基因菌株的初步研究 被引量：13

A preliminary study on the bacteria strain genes with NDM-1 in Ningxia
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摘要 目的掌握宁夏携带I型新德里金属β-内酰胺酶(blaNDM-1)细菌的流行现状。方法用聚合酶链反应(PCR)检测以腹泻病分离保存的菌株,对blaNDM-1检测为阳性的菌株进行生化鉴定、药物敏感性试验,检测blaNDM-1基因测序和16SrRNA序列。结果 2株blaNDM-1基因阳性的菌株均为屎肠球菌,是多重耐药株;blaNDM-1测序结果与国外基因序列比对,同源性为100%。结论宁夏存在携带blaNDM-1基因的革兰氏阳性屎肠球菌。 Objective To investigate the epidemiological status of bacteria carrying New Delhi metallo-β-lactamase-1（NDM-1）in Ningxia.Methods The PCR-DS method was used to detect the bacteria strains isolated from diarrheal patients.Further,the bacteria positive for blaNDM-1 was biochemically analyzed and tested for their sensitivity to antibiotics.Finally,the DNA fragments amplified from the positive bacteria was completely sequenced.Results Two isolates harboring ＂NDM-1＂ were Enterobacteriaceae and they displayed a multidrug resistant phenotype.The DNA sequences exhibited completely identity with the sequences that had been identified in other countries.Conclusion The results show that Ningxia area has Gram positive Enterobacteriaceae carrying NDM-1.
作者 郝琼 刘翔 郭邦成 谢明英 闫立群 王鑫 景怀琦
机构地区 宁夏自治区疾病预防控制中心细菌学检验科 中国疾病预防控制中心传染病预防控制所
出处 《宁夏医学杂志》 CAS 2012年第4期289-291,F0003,共4页 Ningxia Medical Journal
基金 国家"十一五"传染病科技重大专项实验室监测技术平台(2009ZX10004-203)
关键词 聚合酶链反应 屎肠球菌 携带I型新德里金属β-内酰胺酶 基因 PCR Enterococcus faecium BlaNDM-1 Gene
分类号 R440 [医药卫生—诊断学]
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参考文献6

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二级参考文献49

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共引文献20

同被引文献146

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引证文献13

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宁夏医学杂志
2012年 第4期

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