含1,2,3-三氮唑官能团嵌段共聚物的制备及其对蛋白质的吸附

Preparation of 1,2,3-Triazole-Functionalized Polystyrene Diblock Copolymer and Its Adsorption toward Proteins

通过"点击化学"反应合成了含1,2,3-三氮唑官能团的单体(VBTM),再采用可逆加成-断裂链转移(RAFT)活性自由基聚合法,制备了含有1,2,3-三氮唑官能团的聚苯乙烯(PS-b-PVBTM)两嵌段共聚物。将PS-b-PVBTM旋涂于金片表面成膜,形成以聚苯乙烯链段为核、PVBTM链段为冠的胶束结构;此薄膜于90℃退火24 h后,球状结构转变为表面富集PS的环状结构。采用表面等离子体谐振仪(SPR)原位检测蛋白质在PS-b-PVBTM上的吸附动力学。结果表明,当缓冲液为中性时,聚合物薄膜对牛血清蛋白(BSA)和肌红蛋白(Myoglobin)没有吸附,而对等电点为11的溶菌酶(Lysozyme)则有吸附;经退火处理后的聚合物薄膜对Lysozyme的吸附量减少了0.26 ng/mm2;当缓冲液为酸性时,该聚合物薄膜对BSA产生吸附,且吸附量随缓冲液pH值的降低而增加。PS-b-PVBTM对以上3种蛋白质的吸附为静电吸附。

（1-（4-Vinylbenzyl）-1H-1,2,3-triazol-4-yl）methanol（VBTM） monomer was synthesized via click chemistry and RAFT polymerization method was utilized to achieve 1,2,3-triazole-functionalized diblock copolymer（PS-b-PVBTM）.The diblock copolymer was spin-coated on gold substrate to form a thin film consisted of nano-spherical structures with enriched PS as core and PVBTM as corona.After being annealed at 90 ℃ for 24 h,the spherical structure was changed to donut-like nano-structure with PS enriched on the surface.Adsorption of proteins on the spin-coated thin film was studied by surface plasma reasonance.The film could absorb bovine serum albumin（BSA） in an acid buffer solution and the absorption capacity was increased as pH decreased while it did not adsorb BSA and myoglobin in a neutral buffer solution.The film adsorbed lysozyme with a pI of 11.0,and the adsorption capacity was weakened by 0.26 ng/mm2 after being annealed.The electrostatic interaction was supposed to be the governing role of adsorption.