丙烯酰胺-β-环糊精毛细管电色谱手性整体柱的制备及应用

Preparation and Application of Acrylamide-β-cyclodextrin Chiral Monolithic Column for Capillary Electrochromatography

以丙烯酰胺-β-环糊精和聚甲基丙烯酸缩水甘油酯为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用原位聚合法制备了聚丙烯酸酯-β-环糊精手性毛细管整体柱。通过红外和扫描电子显微镜表征了整体柱固定相的结构和形貌。并以苯丙氨酸为考察物考察了柱子的稳定性和重现性,柱效达到47701 plates/m,获得了良好的分离效果。在毛细管电色谱模式下,采用丙烯酰胺-β-环糊精整体柱首次拆分盐酸依替福林对映体,结果使盐酸依替福林对映体达到了基线分离。

With acrylamide-β-cyclodextrin（AM-β-CD） and glycidyl methacrylate acting as functional monomers,and ethylene glycol dimethacrylate acting as cross-linking agent,the chiral capillary monolithic column of acrylamide-β-cyclodextrin was prepared by in situ polymerization method.The structure and morphology of the monolithic column were characterized by IR and scanning electron microscopy（SEM）.Meanwhile,the stability and reproducibility were also studied.The results showed that the capillary monolithic column of acrylamide-β-cyclodextrin had a good separation ability,and the column efficiency of 47701 plates/m was obtained.In capillary electrochromatography（CEC） mode,the etilefrine hydrochloride enantiomers were firstly separated by the chiral capillary monolithic column of acrylamide-β-cyclodextrin.Herein,the baseline resolutions of the enantiomers were completely reached.