冠心病不稳定心绞痛血瘀证患者尿液代谢组学特征的研究

Characteristics of urine metabonomics in patients with blood stasis syndrome of CHD unstable angina

目的利用磁共振波谱代谢学方法研究冠心病不稳定心绞痛血瘀证患者和健康志愿者尿液代谢物的变化。方法通过临床横断面调查研究,收集冠心病不稳定心绞痛血瘀证患者和健康志愿者的一般资料和尿液样品,采用磁共振(NMR)和模式识别技术对磁共振氢谱进行分析,对谱图去水峰后采用0.01化学位移分段积分和归一化处理。所得数据输入SIMCA-P软件,采用偏最小二乘-判别分析法(PLS-DA)对结果进行分析。结果冠心病不稳定心绞痛血瘀证患者尿液中脯氨酸、丙氨酸、异亮氨酸、葡萄糖、缬氨酸、组氨酸、马尿酸等物质的含量升高;柠檬酸、肌酸酐、牛磺酸等物质的含量下降。结合PLS-DA对尿液样品的分析结果显示冠心病患者与健康人在代谢组学方面存在差异,且化合物分布在性别方面也有差异。结论研究表明尿液样品中柠檬酸、脯氨酸、异亮氨酸、牛磺酸等代谢物的改变构成了冠心病不稳定性心绞痛血瘀证患者的代谢组学特征,从而为疾病的诊断和治疗以及中医证候研究提供了新思路和新方法。

Objective To study the changes of urine metabolites in patients with blood stasis syndrome of unstable angina of coronary heart disease（CHD） and health volunteers by applying metabonomics method of nuclear magnetic resonance（NMR）.Methods The general materials and urine samples were collected from the patients（CHD group） and health volunteers（health group） through clinical cross-section survey,and then magnetic resonance spectrogram（MRS） was analyzed with techniques of NMR and pattern recognition technique.The spectrogram after removing water peak was treated with 0.01ppm segmented integral and normalization.All data were imputed into SIMCA-P＋ software（v11.5,Umetrics,Sweden） and the results were analyzed by using partial least square-discriminant analysis（PLS-DA）.Results The content of proline,alanine,isoleucine,glucose,valine,histidine and hippuric acid increased,and content of citric acid,creatine and taurine decreased in the urine samples of CHD group.The analysis of urine samples combined PLS-DA could significantly distinguish CHD group and health group.Conclusion The results of study showed that the changes of citric acid,proline,isoleucine and taurine in urine samples consist of the metabolites features in the patients with blood stasis syndrome of CHD unstable angina,and the study offer a new idea and method for diagnosis,treatment and TCM syndrome research.