摘要
采用紫外分光光度计测定菊叶薯蓣中薯蓣总皂甙含量,确定薯蓣总皂甙的最大吸收波长为480nm,最佳实验条件为:5%香草醛-冰醋酸溶液的加量0.2mL,高氯酸溶液的加量0.8mL,水浴温度70℃,水浴时间15min。精密度实验的相对标准偏差(RSD)为1.25%,加标回收率实验的平均回收率为98.87%。该方法具有操作快捷、精密度高、重现性好等优点。
By UV spectrophotometry the maximum wavelength of dioscin was determined at 480nm,and the optimized experiment conditions were studied： 5% vanillin-glacial acetic acid solution dosage of 0.2ml,high acid solution dosage of 0.8mL,water bath temperature of 70℃,water bath time of 15min.The RSD of precision experiment was 1.12%,and the average recovery of experiment adding standard could be 98.87%.The method was simple,sensitive,and reproduced well.
出处
《食品与发酵科技》
CAS
2012年第2期104-106,共3页
Food and Fermentation Science & Technology
